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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  cDNA_and_Genomic_DNA >  Tissue-Specific_cDNA >  Human_Brain_and_CNS

Tissue-Specific Human cDNA, Brain & CNS

QUICK-Clone cDNAs are premade double-stranded cDNA from which you can amplify a gene of interest using gene-specific primers. QUICK-Clone cDNA is ideal for amplifying previously isolated, structurally related, or cross-species cDNAs. Through direct amplification of a cDNA of interest, traditional library construction and screening can be avoided. QUICK-Clone cDNA can also be used to generate hybridization probes using gene-specific or degenerate primers (1–5).

A high-quality cDNA template is necessary to obtain good results from PCR amplification. Synthesized from premium, high quality poly A+ RNA from various brain and central nervous system tissues using an oligo(dT) primer, QUICK-Clone cDNA is purified to remove residual RNA and size-selected to eliminate cDNA fragments smaller than 400 bp. The result is very pure, double-stranded cDNA ready for PCR.

  At-A-Glance   Documents   Images & Data

Features

  • Highly purified, double-stranded cDNA
  • Clone genes directly by PCR, rather than library screening
  • Prepared from high-quality human tissues
  • Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs

Applications

  • Clone cDNAs without library screening
  • Generate hybridization probes using gene-specific or degenerate primers
  • Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs

References

  1. Parmentier, M., et al. (1989) Science 246:1620–1622.
  2. Wilks, A. E., et al. (1989) Gene 85:67–74.
  3. Vallins, W. J., et al. (1990) FEBS Letters 270:57–61.
  4. Lee, C. C., et al. (1998) Science 239:1288–1291.
  5. Schuchman, E. H., et al. (1990) Genomics 6:149–158.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
637239 Human Bone Marrow QUICK-Clone™ cDNA 2 x 10 Rxns $552.00
637244 Human Brain, Amygdala QUICK-Clone™ cDNA 2 x 10 Rxns $618.00
637212 Human Brain, Cerebellum QUICK-Clone™ cDNA 2 x 10 Rxns $618.00
637202 Human Brain, Cerebral Cortex QUICK-Clone™ cDNA 2 x 10 Rxns $618.00
637228 Human Brain, Hippocampus QUICK-Clone™ cDNA 2 x 10 Rxns $618.00
637243 Human Brain, Thalamus QUICK-Clone™ cDNA 2 x 10 Rxns $618.00
637242 Human Brain, Whole QUICK-Clone™ cDNA 2 x 10 Rxns $618.00
637232 Human Pituitary Gland QUICK-Clone™ cDNA 2 x 10 Rxns $552.00
637222 Human Spinal Cord QUICK-Clone™ cDNA 2 x 10 Rxns $618.00
 

 

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