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cDNA Synthesis & Library Construction

Products >  cDNA_Synthesis_and_Library_Construction >  cDNA_and_Genomic_DNA >  RACE-Ready_cDNA >  Human_Internal_Organ

RACE-Ready Human cDNA, Internal Organ

Marathon-Ready cDNAs—cDNAs made from high-quality Premium Poly A+ RNA and ligated to the Marathon Adaptor—are ready for 5'- and 3'-RACE PCR (1). Each Marathon-Ready cDNA is a premade, tissue-specific “pool” of double-stranded cDNA from which full-length genes can be amplified by using sets of gene-specific primers. They can also be used to study tissue-specific gene expression and to find polymorphic forms of mRNA or mRNA belonging to a multigene family.

Marathon-Ready cDNA is synthesized from Premium RNA prepared from various internal organ tissues using a procedure optimized to produce full-length cDNA and to eliminate 3' heterogeneity (2, 3). After synthesis, blunt ends are created and the Marathon Adaptor is ligated to both ends of the double-stranded cDNA.

  At-A-Glance   Documents   Images & Data


  • Adaptor-ligated and ready for RACE PCR
  • Tissue-specific "pool" of double-stranded cDNA
  • Obtain full-length cDNAs without ever constructing or screening a cDNA library
  • Perfect template for Marathon cDNA Amplification


  • Rapid amplification of cDNA ends (RACE)
  • Study tissue-specific gene expression
  • Discover polymorphic forms of mRNA
  • Obtain full-length copies of published cDNAs
  • Characterization of tissue-specific patterns of gene expression


For current source information, please refer to the Certificate of Analysis accompanying each Marathon-Ready cDNA.


1. Chenchik, A., et al. (1996) BioTechniques 21:526–532.
2. Chenchik, A., et al. (January 1995) Clontechniques X(1):5–8.
3. Borson, N. D., et al. (1992) PCR Methods Appl. 2:144–148.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Cat. # Product Contents Size Price Units Select
639331 Human Colon Marathon®-Ready cDNA 30 Rxns $748.00
639305 Human Kidney Marathon®-Ready cDNA 30 Rxns $748.00
639307 Human Liver Marathon®-Ready cDNA 30 Rxns $748.00
639308 Human Lung Marathon®-Ready cDNA 30 Rxns $748.00
639310 Human Pancreas Marathon®-Ready cDNA 30 Rxns $748.00
639349 Human Retina Marathon®-Ready cDNA 30 Rxns $748.00
639313 Human Skeletal Muscle Marathon®-Ready cDNA 30 Rxns $748.00
639326 Human Small Intestine Marathon®-Ready cDNA 30 Rxns $748.00
639312 Human Spleen Marathon®-Ready cDNA 30 Rxns $748.00
639327 Human Stomach Marathon®-Ready cDNA 30 Rxns $748.00
639315 Human Thymus Marathon®-Ready cDNA 30 Rxns $748.00
639350 Human Thyroid Gland Marathon®-Ready cDNA 30 Rxns $748.00


Required Products
Cat. # Product Contents Size Price # of Units Select
634913 Marathon® cDNA Amplification Kit 100 Rxns $759.00


Recommended Products
Offers Cat. # Product Contents Size Price Units Select
  639206 Advantage® 2 PCR Kit 100 Rxns $432.00
639208 Titanium® Taq DNA Polymerase 100 Rxns $143.00

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