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Products >  cDNA_Synthesis_and_Library_Construction >  cDNA_and_Genomic_DNA >  RACE-Ready_cDNA >  Human_Cancer_and_Cell_Line

RACE-Ready Human cDNA, Cancer & Cell Line

Marathon-Ready cDNAs—cDNAs made from high-quality Premium Poly A+ RNA and ligated to the Marathon Adaptor—are ready for 5'- and 3'-RACE PCR (1). Each Marathon-Ready cDNA is a premade, tissue-specific “pool” of double-stranded cDNA from which full-length genes can be amplified by using sets of gene-specific primers. They can also be used to study tissue-specific gene expression and to find polymorphic forms of mRNA or mRNA belonging to a multigene family.

Marathon-Ready cDNA is synthesized from Premium RNA prepared from cancer tissues and cancer cell lines using a procedure optimized to produce full-length cDNA and to eliminate 3' heterogeneity (2, 3). After synthesis, blunt ends are created and the Marathon Adaptor is ligated to both ends of the double-stranded cDNA.
  At-A-Glance   Documents   Images & Data

Features

  • Adaptor-ligated and ready for RACE PCR
  • Tissue-specific "pool" of double-stranded cDNA
  • Obtain full-length cDNAs without ever constructing or screening a cDNA library
  • Perfect template for Marathon cDNA Amplification

Applications

  • Rapid amplification of cDNA ends (RACE)
  • Study tissue-specific gene expression
  • Discover polymorphic forms of mRNA
  • Obtain full-length copies of published cDNAs
  • Characterization of tissue-specific patterns of gene expression

Source

For current source information, please refer to the Certificate of Analysis accompanying each Marathon-Ready cDNA.

References

  1. Chenchik, A., et al. (1996) BioTechniques 21:526–532.
  2. Chenchik, A., et al. (January 1995) Clontechniques X(1):5–8.
  3. Borson, N. D., et al. (1992) PCR Methods Appl. 2:144–148.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
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639339 Human HeLa Marathon®-Ready cDNA 30 Rxns $719.00
 

 

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639208 Titanium® Taq DNA Polymerase 100 Rxns $143.00 License Statements
 

 

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