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Products >  cDNA_Synthesis_and_Library_Construction >  cDNA_and_Genomic_DNA >  RACE-Ready_cDNA >  Human_Blood_and_Immune_System

RACE-Ready Human cDNA, Blood & Immune System

Marathon-Ready cDNAs—cDNAs made from high-quality Premium Poly A+ RNA and ligated to the Marathon Adaptor—are ready for 5'- and 3'-RACE PCR (1). Each Marathon-Ready cDNA is a premade, tissue-specific “pool” of double-stranded cDNA from which full-length genes can be amplified by using sets of gene-specific primers. They can also be used to study tissue-specific gene expression and to find polymorphic forms of mRNA or mRNA belonging to a multigene family.

Marathon-Ready cDNA is synthesized from Premium RNA prepared from blood and immune system tissues and cells using a procedure optimized to produce full-length cDNA and to eliminate 3' heterogeneity (2, 3). After synthesis, blunt ends are created and the Marathon Adaptor is ligated to both ends of the double-stranded cDNA.

At-A-Glance Documents Images & Data

Features

  • Adaptor-ligated and ready for RACE PCR
  • Tissue-specific "pool" of double-stranded cDNA
  • Obtain full-length cDNAs without ever constructing or screening a cDNA library
  • Perfect template for Marathon cDNA Amplification

Applications

  • Rapid amplification of cDNA ends (RACE)
  • Study tissue-specific gene expression
  • Discover polymorphic forms of mRNA
  • Obtain full-length copies of published cDNAs
  • Characterization of tissue-specific patterns of gene expression

Source

For current source information, please refer to the Certificate of Analysis accompanying each Marathon-Ready cDNA.

References

1. Chenchik, A., et al. (1996) BioTechniques 21:526–532.
2. Chenchik, A., et al. (January 1995) Clontechniques X(1):5–8.
3. Borson, N. D., et al. (1992) PCR Methods Appl. 2:144–148.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
639325 Human Aorta Marathon®-Ready cDNA 30 Rxns $677.00 License Statements
639316 Human Bone Marrow Marathon®-Ready cDNA 30 Rxns $677.00 License Statements
639304 Human Heart Marathon®-Ready cDNA 30 Rxns $677.00 License Statements
639306 Human Leukocyte Marathon®-Ready cDNA 30 Rxns $677.00 License Statements
 

 

Required Products
Cat. # Product Package Size Price # of Units Select
634913 Marathon® cDNA Amplification Kit 5 cDNA & 100 PCR Rxns $759.00
 

 

 


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