Genome Walking—GenomeWalker Kits—Find and Identify a Gene of Interest
GenomeWalker Kits provide a simple, PCR-based method for walking upstream or downstream in genomic DNA from a known sequence, such as an expressed sequence tag (EST), in order to find unknown sequences. GenomeWalker allows you to take individual steps of up to 6 kb in genomic DNA using long-distance PCR. Walks can be extended simply by taking multiple steps, using new primers based on the sequence obtained in previous steps. The Universal GenomeWalker 2.0 Kit contains all the reagents needed to construct GenomeWalker libraries and perform PCR-based “walking” in the genomic DNA of any species. In addition to library construction components, it includes a thermostable DNA polymerase mix for PCR, and materials for the purification of genomic DNA and clean-up of digested DNA. The GenomeWalker Universal 2.0 kit supplies reagents sufficient to generate libraries from three different samples and to perform 80 walks, using a set of gene-specific primers (GSP) that you provide.