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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  cDNA_and_Genomic_DNA >  GenomeWalker_Kits

Genome Walking—GenomeWalker Kits—Find and Identify a Gene of Interest

GenomeWalker Kits provide a simple, PCR-based method for walking upstream or downstream in genomic DNA from a known sequence, such as an expressed sequence tag (EST), in order to find unknown sequences. GenomeWalker allows you to take individual steps of up to 6 kb in genomic DNA using long-distance PCR. Walks can be extended simply by taking multiple steps, using new primers based on the sequence obtained in previous steps. The Universal GenomeWalker 2.0 Kit contains all the reagents needed to construct GenomeWalker libraries and perform PCR-based “walking” in the genomic DNA of any species. In addition to library construction components, it includes a thermostable DNA polymerase mix for PCR, and materials for the purification of genomic DNA and clean-up of digested DNA. The GenomeWalker Universal 2.0 kit supplies reagents sufficient to generate libraries from three different samples and to perform 80 walks, using a set of gene-specific primers (GSP) that you provide.

At-A-Glance Documents Images & Data

Features

  • Rapid PCR-based walking in genomic DNA
  • Ideal for obtaining exon-intron junctions, promoters, or any other regulatory element
  • Rapidly determine unknown sequences

Applications

  • Genomic DNA walking
  • Obtain promoters or regulatory sequences
  • Map intron/exon junctions
  • Determine integration sites for transposons and viruses
  • Confirm genome modifications performed using zinc finger nucleases, TAL effector nucleases, or other methods
  • Genomic DNA cloning

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
636405 Universal GenomeWalker™ 2.0 Each $778.00 License Statements
 

 

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