Gene expression data obtained with the SMARTer Ultra Low RNA Kit with 1 ng total RNA
Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR. Scatter plots were used to compare differential expression data obtained by sequencing with the SMARTer Ultra Low RNA Kit (1 ng total RNA) and quantitative PCR (qPCR) data available for Universal Human Reference Total RNA (UHR) and Human Brain Reference RNA through the MicroArray Quality Control (MAQC) project. The differential expression of ~700 genes showed correlation values of 0.94, demonstrating that the sequencing results are consistent with orthogonal gene expression technologies.