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Products >  cDNA_Synthesis_and_Library_Construction >  RNA >  RNA_cDNA_Quality_Assay

RNA & cDNA Quality Assay

The Clontech RNA/cDNA Quality Assay Kit directly determines the quality of your human RNA and cDNA samples using reverse transcription (RT) and PCR. To ensure the representation of full-length transcripts for experiments with cDNA libraries, RACE-ready cDNAs, or cDNA microarray probes, use the Clontech RNA/cDNA Quality Assay to check the integrity of your RNA starting material before beginning your experiment. Because this assay uses RT-PCR, it provides a direct functional test of your sample for its ability to produce full-length cDNA for your application. You achieve quick results using standard lab equipment, and avoid inconvenient and toxic formaldehyde gels.

Analyze RNA and cDNA Integrity

While RNA can look intact on a formaldehyde gel or in a chip-based assay (28S:18S ratio >1), RNA can be degraded during cDNA synthesis. The result: first-strand cDNA containing a mix of truncated and full-length transcripts.

Using our RNA/cDNA Quality Assay ensures that you won’t waste time or get invalid results by using low-quality RNA in demanding applications. The assay uses two sets of primers in a multiplex RT-PCR to amplify 5'- and 3'-end fragments of a long mRNA (~3 kb) for a carefully selected housekeeping gene. Since in most cases RNA degradation starts in the 5'-end region of an RNA molecule, the ratio of the 3' to 5' amplified fragments provides a direct indication of RNA integrity.

At-A-Glance Documents Images & Data

Features

Simple PCR-based alternative for assessing RNA & cDNA quality:

  • Start with total RNA, poly A+ RNA, or first-strand cDNA
  • Assess samples for functional ability to produce full-length transcripts
  • Start with as little as 100 ng total RNA
  • Non-toxic, user-friendly, and fast
  • No special equipment is required

Applications

  • Quality determination of human or mouse RNA or cDNA sample
  • Determine the ability to produce full-length cDNA from your sample

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
636841 RNA/cDNA Quality Assay 40 Assays $434.00 License Statements
 

 

 


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