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Products >  cDNA_Synthesis_and_Library_Construction >  RACE_Rapid_Amplification_of_cDNA_Ends >  5_Prime_RACE_and_3_Prime_RACE

cDNA Amplification Kits for Rapid Amplification of cDNA Ends—5' RACE & 3' RACE

Rapid Amplification of cDNA Ends (RACE) is a technique used to obtain the full-length sequence of an RNA transcript. RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript all the way to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA.

The SMARTer RACE 5’/3’ Kit is an improved version of the SMARTer RACE cDNA Amplification Kit. The SMARTer RACE 5’/3’ Kit provides improved sensitivity, less background and higher specificity over the previous generation of kits. The first-strand cDNA generated with this kit can be used directly in 5’- and 3’-RACE PCR reactions. The SMARTer protocol does not involve any of the adaptor ligation steps that other RACE kits incorporate, making the protocol shorter and significantly easier to use. The new kit is also designed to accommodate larger RNA input volumes (up to 11 µl) and has been shown to perform more efficiently on challenging targets (e.g., those that are long, GC-rich, etc.). The kit includes our highly robust SeqAmp DNA Polymerase for amplification of products, and the In-Fusion HD Cloning Kit for efficient cloning of RACE fragments in the provided linearized vector. The SMARTer RACE 5’/3’ procedure combines SMART first-strand cDNA synthesis technology with a powerful suppression PCR protocol that vastly reduces the background amplification commonly associated with RACE protocols.

  • Easy to Use
    The SMARTer RACE protocol is performed in a single tube, and minimizes handling of both your RNA sample and your synthesized cDNA. Total hands-on time is only four hours.
  • Complete Kit
    The SMARTer RACE 5’/3’ Kit contains all the necessary components (save gene-specific primers) to streamline your process. With this single kit, you can begin first-strand cDNA synthesis and proceed through cloning RACE fragments, recovering successful clones on day two.
  • Requires Only 10 ng of Total RNA
    Our SMARTer RACE method allows you to utilize small samples, including biopsies, tissue dissections, needle aspirations, and embryonic and rare disease tissues. This optimized protocol significantly reduces non-specific background, and such a reduction is essential when handling very small sample sizes.
  • Specific Enrichment for 5' Ends
    Our carefully designed, chemically-treated SMARTer Oligo preferentially hybridizes to the 5' ends of the cDNA being synthesized. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5' sequences, thus increasing the likelihood you will amplify the entire sequence of your gene or the upstream regulatory regions.
  • No RNA Pretreatment Required
    Our SMARTer RACE method requires no RNA pretreatment with DNase. This protocol works with total RNA, as well as with samples that may be contaminated with genomic DNA.

Related Products

Marathon cDNA Amplification Kit

The Marathon cDNA Amplification Kit method employs a specially-designed adaptor that significantly reduces background and permits both 5'- and 3'-RACE reactions (1, 2) to be performed using the same template. Marathon cDNA amplification can be used to quickly characterize multiple RNAs identified by expressed sequence tags (ESTs), differential display, RNA fingerprinting, or cDNA subtraction. Marathon cDNA synthesis begins with poly A+ RNA and a modified lock-docking oligo(dT) primer that contains two degenerate nucleotides at the 3' end. These nucleotides position the primer at the beginning of the poly A+ tail, eliminating the 3' heterogeneity inherent with conventional oligo(dT) priming. Following cDNA synthesis, blunt ends are created and the Marathon Adaptor is ligated to both ends of the double-stranded cDNA.

NOTE: The Marathon cDNA Amplification Kit requires the Advantage 2 PCR Kit (Cat. No. 639206).

RACE-READY cDNAs

Clontech also offers RACE-Ready cDNAs, which are double stranded cDNAs made from high-quality Premium Poly A+ RNA and ligated to the Marathon Adaptor. These cDNAs are ready for 5'- and 3'-RACE PCR and are available from a wide range of tissues and cell types.

  At-A-Glance   Documents   Images & Data   Resources

Features

SMARTer RACE 5'/3' Kit

  • Simple kit—easy to use
  • SeqAmp DNA Polymerase provides robust PCR performance
  • In-Fusion HD technology enables fast, easy cloning of RACE fragments
  • Requires Only 10 ng of total RNA
  • Specific enrichment for either 5' or 3' ends
  • No RNA pretreatment required

Marathon cDNA Amplification Kit

  • Quickly obtain cDNAs without library screening
  • Avoid troublesome single-stranded ligation or tailing reactions
  • Perform 5' and 3' RACE from the same template

Applications

  • RACE (Rapid Amplification of cDNA Ends)
  • Identification of 3'/5' UTRs
  • Full-length gene cloning
  • Gene cloning and analysis

References

  1. Bertling, W. M., et al. (1993) PCR Methods Appl. 3:95–99.
  2. Frohman, M. A. (1991) Methods Enzymol. 218:340–362.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
  634913 Marathon® cDNA Amplification Kit 5 cDNA & 100 PCR Rxns $759.00 License Statements
634858 SMARTer® RACE 5’/3’ Kit 10 Rxns $650.00 License Statements
634859 SMARTer® RACE 5’/3’ Kit 20 Rxns $1,100.00 License Statements
  634922 Universal Primer Mix 100 Rxns $126.00  
 

 

 
 

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