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Products >  cDNA_Synthesis_and_Library_Construction >  Oligo_Modification >  Label-ON_Reagents

Oligo Modification—Label-ON Reagents

Label-ON Reagents provide an efficient way to directly label oligonucleotides with various reporter molecules (1–4). All Label-ON Reagents possess the patented 2-aminobutyl-1,3-propanediol (ABPD) backbone, which offers the following distinct labeling advantages:

  • Multiple labels can be incorporated because Label-ON Phosphoramidites do not truncate chain elongation at the 5' terminus.
  • Natural 3-carbon internucleotide phosphodiester distance is maintained, providing better hybridization and annealing properties than those obtained with other labeling methods.
  • Attached label is separated from the phosphodiester connection point to reduce steric hindrance. The ABPD backbone has a 7-atom spacer arm that separates the label from the connection point.
  • Label incorporation can be easily measured by standard trityl cation assays because all Label-ON Phosphoramidites possess a dimethoxytrityl (DMT) protected hydroxyl group.

The Uni-Link AminoModifier allows you to directly incorporate a primary amine onto the 5' end of an oligonucleotide using automated synthesis. All Label-ON reagents are provided with a User Manual.

Please inquire about bulk orders or custom synthesis requests at tech@clontech.com.

  At-A-Glance   Documents   Images & Data

Features

  • Incorporate single or multiple labels during oligonucleotide synthesis
  • Place labels at the 5' end, internally, or at the 3' end
  • Maintain natural 3-carbon internucleotide phosphate distance
  • Obtain very high coupling efficiencies on any commercially available synthesizer

Applications

  • Oligo labeling (reagents)
  • Nucleic acid modifications
  • Nucleic acid chemistry (reagents)
  • Nucleic acid labeling (reagents)
  • Nucleic acid synthesis (reagents)

References

  1. Nelson, P., et al. (1992) Nucleic Acids Res. 20(23):6253–6259.
  2. Nelson, P. & Muthini, S. (1993) OligoTechniques 1(1):1–4.
  3. Nelson, P., et al. (1993) OligoTechniques 1(1):5–7.
  4. Nelson, P. & Muthini, S. (1993) Biomedical Products 18(6):40.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Size Price Units Select
635703 Uni-Link™ AminoModifier 100 mg $169.00
 

 

 
 

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