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Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  smRNA-Seq

SMARTer smRNA-Seq Kit for Illumina—Sequence Small RNAs with High Sensitivity and Minimal Bias

Discovery and analysis of small non-coding RNAs (smRNAs) has become an important part of understanding gene expression regulation. Some of the well-known small RNA species are miRNAs, siRNAs, piRNAs, and snoRNAs, many of which range in size from 20 to 30 nt. The SMARTer smRNA-Seq Kit for Illumina has been specifically designed to generate high-quality Illumina-ready sequencing libraries from low-input amounts of small RNAs such as these.

This kit was developed to work directly from 1 ng–2 µg inputs of total RNA or enriched small RNA samples, and incorporates features from the industry-leading SMART-Seq v4 kit, including SMART technology (Switching Mechanism at 5’ End of RNA Template) and locked nucleic acids (LNAs). Libraries are generated in a ligation-free manner, ensuring that diverse small RNA species are represented with minimal bias.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Analysis of diverse small RNA species—Sequencing library prep of small RNAs ranging in size from 15–150 nt
  • Flexible sample inputs—Start from 1 ng–2 µg of total RNA or enriched small RNA
  • Illumina-ready sequencing libraries—Ligation-free incorporation of Illumina adapters and indexes for sample multiplexing
  • Quick, single-tube workflow—Generate sequencing libraries in ~3 hours (not including validation and post-PCR size-selection steps)

Applications

  • Illumina-ready sequencing library preparation, specifically for small RNAs ranging in size from 15–150 nt

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
635029 SMARTer® smRNA-Seq Kit for Illumina® 12 Rxns $720.00 License Statements
635030 SMARTer® smRNA-Seq Kit for Illumina® 48 Rxns $2,448.00 License Statements
635031 SMARTer® smRNA-Seq Kit for Illumina® 96 Rxns $4,416.00 License Statements
 

 

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