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Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Universal_RNA_Seq_Random_Primed

SMARTer Universal Low Input RNA Kit—cDNA Synthesis for NGS from Degraded Samples

The SMARTer Universal Low Input RNA Kit for Sequencing provides a simple and efficient solution for generating cDNA for RNA-seq from very low amounts of input RNA that is compromised, has low RIN values, or is derived from a source that does not have polyadenlyated mRNA. The kit works with as little as 200 pg of rRNA-depleted material (derived from 2–100 ng of total RNA). The SMARTer Universal Low Input RNA Kit for Sequencing has been designed specifically for RNA-seq with FFPE samples and laser captured materials. It outperforms existing methods in body coverage, reproducibility, mappability and ERCC correlation.

At-A-Glance Documents Images & Data Resources

Features

  • Sensitivity to handle low-input samples—Works with as little as 200 pg of input RNA rRNA-depleted material (derived from 2–100 ng of total RNA)
  • Efficient results from compromised samples—Ideal solution for FFPE samples
  • RNA-seq data quality—Consistent RNA-seq data across all input ranges (200 pg–10 ng)
    • Mappability > 80%
    • Reproducibility >0.98
    • ERCC correlation > 0.99
    • Number of genes > 15,000
  • Complete kit includes RT and PCR enzymes
  • Catalog numbers 634945 and 634946 contain compatible Low input library preparation kit for Illumina platforms.

Applications

  • Generate cDNA for RNA-seq from very low amounts of total RNA (2–10 ng) from FFPE or LCM samples
  • Generate cDNA for NGS  from very low amounts of total RNA (2–10 ng) that are:
    • Compromised (obtained from FFPE or LCM samples)
    • Have low RIN values (<8)
    • Are from sources that do not have polyadenylated mRNA

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Package Size Price License # of Units Select
634938 SMARTer® Universal Low Input RNA Kit for Sequencing 10 Rxns $1,349.00 License Statements
634940 SMARTer® Universal Low Input RNA Kit for Sequencing 25 Rxns $2,730.00 License Statements
634945 SMARTer® Universal Low Input RNA Library Prep Kit 10 Rxns $2,190.00 License Statements
634946 SMARTer® Universal Low Input RNA Library Prep Kit 25 Rxns $4,400.00 License Statements
 

 

Required Products
Cat. # Product Package Size Price # of Units Select
634947 Low Input Library Prep Kit 12 Rxns $1,140.00
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
639207 Advantage® 2 PCR Kit 30 Rxns $135.00
 
 


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