SMARTer Universal Low Input RNA Kit—cDNA Synthesis for NGS from Degraded Samples
The SMARTer Universal Low Input RNA Kit for Sequencing provides a simple and efficient solution for generating cDNA for RNA-seq from very low amounts of input RNA that is compromised, has low RIN values, or is derived from a source that does not have polyadenlyated mRNA. The kit works with as little as 200 pg of rRNA-depleted material (derived from 2–100 ng of total RNA). The SMARTer Universal Low Input RNA Kit for Sequencing has been designed specifically for RNA-seq with FFPE samples and laser captured materials. It outperforms existing methods in body coverage, reproducibility, mappability and ERCC correlation. cDNA produced with this kit is compatible with both Illumina® and Ion Torrent low-input library preparation kits.