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Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Total_RNA-Seq >  Universal_RNA_Seq_Random_Primed

SMARTer Universal Low Input RNA Kit—cDNA Synthesis for NGS from Degraded Samples

The SMARTer Universal Low Input RNA Kit for Sequencing provides a simple and efficient solution for generating cDNA for RNA-seq from very low amounts of input RNA that is compromised, has low RIN values, or is derived from a source that does not have polyadenlyated mRNA. The kit works with as little as 200 pg of rRNA-depleted material (derived from 2–100 ng of total RNA). The SMARTer Universal Low Input RNA Kit for Sequencing has been designed specifically for RNA-seq with FFPE samples and laser captured materials. It outperforms existing methods in body coverage, reproducibility, mappability and ERCC correlation. cDNA produced with this kit is compatible with both Illumina® and Ion Torrent low-input library preparation kits. 

For rRNA removal from 10–100 ng of input total RNA, we recommend the RiboGone - Mammalian kit. For 2–10 ng of input total RNA, we recommend using a modified protocol with Ribo-Zero™ technology from Illumina

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Sensitivity to handle low-input samples—Works with as little as 200 pg of input RNA rRNA-depleted material (derived from 2–100 ng of total RNA)
  • Efficient results from compromised samples—Ideal solution for FFPE samples
  • RNA-seq data quality—Consistent RNA-seq data across all input ranges (200 pg–10 ng)
    • Mappability > 80%
    • Reproducibility >0.98
    • ERCC correlation > 0.99
    • Number of genes > 15,000
  • Complete kit includes RT and PCR enzymes
  • Use the table below to choose the right kit:
cDNA Synthesis KitRNA-Seq Library Preparation
SMARTer Universal Low Input RNA Kit for Sequencing (Cat. Nos. 634938, 634940)

Ion Torrent or Illumina kits (not included)
SMARTer Universal Low Input RNA Library Prep Kit (Cat. Nos. 634945, 634946)Low Input Library Prep Kit included (for Illumina sequencing only)

Interested in more data and FAQs about this product? Visit the NGS Resource Portal

Applications

  • Generate cDNA for RNA-seq from very low amounts of total RNA (2–10 ng) from FFPE or LCM samples
  • Generate cDNA for NGS  from very low amounts of total RNA (2–10 ng) that are:
    • Compromised (obtained from FFPE or LCM samples)
    • Have low RIN values (<8)
    • Are from sources that do not have polyadenylated mRNA

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
634938 SMARTer® Universal Low Input RNA Kit for Sequencing 10 Rxns $1,349.00 License Statements
634940 SMARTer® Universal Low Input RNA Kit for Sequencing 25 Rxns $2,730.00 License Statements
634945 SMARTer® Universal Low Input RNA Library Prep Kit 10 Rxns $2,190.00 License Statements
634946 SMARTer® Universal Low Input RNA Library Prep Kit 25 Rxns $4,400.00 License Statements
 

 

Required Products
Cat. # Product Contents Size Price # of Units Select
634947 Low Input Library Prep Kit 12 Rxns $1,140.00
 

 

Recommended Products
New Cat. # Product Contents Size Price Units Select
  639207 Advantage® 2 PCR Kit 30 Rxns $135.00
634846 RiboGone™ - Mammalian 6 Rxns $480.00
634847 RiboGone™ - Mammalian 24 Rxns $1,680.00
638325 DNA Standards for Library Quantification 50 Rxns $225.00
638324 Library Quantification Kit 500 Rxns $515.00
 
 
 

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