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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Total_RNA-Seq >  Pico_Input_Total_RNA_Seq_Illumina

SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian
Strand-Specific RNA-Seq Libraries for Illumina Platforms from Picogram Inputs of Total RNA

The SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian is designed for efficient preparation of Illumina® sequencing libraries from picogram input amounts (250 pg–10 ng) of total RNA. The technologies in this product are designed to process high-quality, partially degraded, and low-quality (e.g., prepared from FFPE or LCM samples) RNA. The kit has a streamlined workflow that retains strand information and eliminates downstream library prep by incorporating indexes and adapters during the reverse-transcription and PCR-amplification steps.

Ribosomal RNA (rRNA) comprises a significant proportion (~90%) of total RNA samples. Depleting these abundant transcripts from total RNA prior to generating libraries provides benefits by lowering sequencing costs and improving mapping statistics. However, with very low input amounts, initial rRNA depletion from total RNA leaves an insufficient amount of material for the preparation of a good-quality library. The workflow used in this kit incorporates a proprietary technology that depletes ribosomal cDNA (cDNA fragments originating from rRNA molecules) using probes specific to mammalian rRNA and some mitochondrial RNA.

The high-throughput version of the kit (Cat. # 635007) contains 12 reverse primers and 8 forward primers, allowing library construction with up to 96 separate indexes. The specific sequences of the primers depend on the reaction size (version) of the kit you choose. The entire protocol, including library prep and purification, takes only ~5 hours.

Alternate kits for other input amounts:

  At-A-Glance   Documents   Images & Data

Features

  • Allows picogram input amounts of starting material—Use 250 pg–10 ng total RNA from human, mouse, or rat
  • Versatile—Get reproducible data from a range of sample tissue types (including FFPE and LCM samples) and from RNA of varying quality
  • Stranded information—Identify each transcript’s strand of origin with very high accuracy
  • Fast, streamlined protocol—Go from start to finish in ~5 hours
  • Seamless integration with Illumina sequencing—Generate Illumina-ready libraries with up to 96 indexes

Interested in more data and FAQs about this product? Visit the NGS Resource Portal.

Applications

  • Robust NGS library generation that retains strand information
  • Use for RNA-seq on Illumina platforms
  • Captures coding and non-coding information from total mammalian RNA

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
635005 SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian 12 Rxns $1,200.00 License Statements
635006 SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian 48 Rxns $3,650.00 License Statements
635007 SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian 96 Rxns $4,800.00 License Statements
 

 

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