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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Total_RNA-Seq >  High_Input_Total_RNA_Seq_Illumina

SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian—Strand-Specific RNA-Seq Libraries for Illumina Platforms from High-Input Total RNA

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina® sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines key RiboGone and SMART technologies, seamlessly blending efficient rRNA removal and strand-specific library generation in around 5 hours. Because rRNA comprises ~90% of total RNA, effective depletion of these abundant transcripts prior to library generation can lower sequencing costs and improve mapping statistics. The core SMART technology completes a streamlined process that maintains strand information, and eliminates downstream library prep by incorporating indexes and adapters during the reverse transcription and PCR amplification steps.

These high-input kits contain 12 Reverse Primers and up to 8 Forward Primers to generate up to 96 separate indexes in HT format. The specific primers you receive will depend on your particular kit. The entire protocol—from rRNA depletion through creation of Illumina-specific, indexed RNA-seq libraries—takes only ~5 hours.

Alternate kits for other input amounts:

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Strand information—Identify each transcript’s strand of origin with >99% accuracy.
  • Fast, streamlined protocol—Go from start to finish in ~5 hours.
  • Large input size—Use 100 ng–1 µg total RNA from human, mouse, or rat.
  • Versatility—Get reliable data across replicates and RNA quality. See high-quality, reproducible data from total RNA with a RIN (RNA Integrity Number) from 3–10.
  • Integrated with Illumina sequencing—Generate Illumina-specific libraries with up to 96 indexes, and retain strand information.

Interested in more data and FAQs about this product? Visit the NGS Resource Portal

Applications

  • Illumina-specific NGS library generation that retains strand information
  • RNA-Seq on Illumina platforms for mammalian samples
  • Coding and non-coding transcript information

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
634873 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 12 Rxns $1,440.00 License Statements
634874 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 24 Rxns $2,448.00 License Statements
634876 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 96 Rxns $7,075.00 License Statements
634877 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 192 Rxns $11,320.00 License Statements
634878 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 480 Rxns $22,369.00 License Statements
634875 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 48 Rxns $4,162.00 License Statements
 

 

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