SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian—Strand-Specific RNA-Seq Libraries for Illumina Platforms from High-Input Total RNA
The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina® sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines key RiboGone and SMART technologies, seamlessly blending efficient rRNA removal and strand-specific library generation in around 5 hours. Because rRNA comprises ~90% of total RNA, effective depletion of these abundant transcripts prior to library generation can lower sequencing costs and improve mapping statistics. The core SMART technology completes a streamlined process that maintains strand information, and eliminates downstream library prep by incorporating indexes and adapters during the reverse transcription and PCR amplification steps.
These high-input kits contain 12 Reverse Primers and up to 8 Forward Primers to generate up to 96 separate indexes in HT format. The specific primers you receive will depend on your particular kit. The entire protocol—from rRNA depletion through creation of Illumina-specific, indexed RNA-seq libraries—takes only ~5 hours.