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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Strand-Specific_RNA_Seq_Illumina

SMARTer Stranded RNA-Seq Kits—Strand-Specific Library Construction for Transcriptome Analysis on Illumina Platforms

The SMARTer Stranded RNA-Seq Kit provides a solution for generating libraries for Illumina® sequencing platforms that retain strand information with >99% accuracy. This kit generates robust data with input as low as 100 pg to 100 ng of RNA. The SMARTer Stranded RNA-Seq Kit uses a random priming method that is applicable to both polyA RNA and rRNA-removed total RNA. The kit can be used with both mammalian RNA and bacterial samples. The core SMART technology has been modified in this kit to generate complete transcriptome coverage of both coding and non-coding RNA while incorporating indexes and adapters in the workflow, which eliminates the need for a downstream library preparation kit. You can generate indexed libraries that are ready for Illumina sequencing in less than 4 hours using the SMARTer Stranded RNA-Seq kit protocol. We also offer the SMARTer Stranded RNA-Seq Kit HT includes 8 Forward Primers and 12 Reverse Primers to generate up to 96 separate indexes for high-throughput reactions.

For rRNA removal from 10–100 ng of input total RNA, we recommend the RiboGone - Mammalian kit. The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian combines SMARTer Stranded technology with RiboGone rRNA removal for complete, seamless RNA-seq library preparation from total RNA samples in <5 hours.

For 2–10 ng of input total RNA, we recommend using a modified protocol with Ribo-Zero™ technology from Illumina.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Accurate—Identify each transcript's strand of origin with >99% accuracy.
  • Sensitive—Applicable to as low as 100 pg to 100 ng of input RNA.
  • Integrated with Illumina sequencing—Incorporates indexes and Illumina adaptors in the workflow.
  • Couldn't get easier—Start to finish in less than 4 hours.
  • SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian—(Cat. No. 634861) A complete solution from total RNA to RNA-seq libraries for most human, rat or mouse sample types.
  • SMARTer Stranded RNA-Seq Kit HT—(Cat. No. 634862) Uses Illumina high-throughput indexes to generate up to 96 indexed RNA-seq libraries. All other SMARTer Stranded RNA-Seq Kits utilize 12 low-throughput indexes.

Applications

  • Illumina-specific NGS library production that retains strand information.
  • RNA-Seq on Illumina platforms for both mammalian and prokaryotic samples.
  • Coding and non-coding transcript information.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
634836 SMARTer® Stranded RNA-Seq Kit 12 Rxns $1,200.00 License Statements
634837 SMARTer® Stranded RNA-Seq Kit 24 Rxns $2,160.00 License Statements
634838 SMARTer® Stranded RNA-Seq Kit 48 Rxns $3,650.00 License Statements
634839 SMARTer® Stranded RNA-Seq Kit 96 Rxns $4,700.00 License Statements
634861 SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian 24 Rxns $3,380.00 License Statements
634862 SMARTer® Stranded RNA-Seq Kit HT 96 Rxns $4,800.00 License Statements
 

 

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638509 SeqAmp™ DNA Polymerase 200 Rxns $295.00
 
 
 

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