SMARTer Stranded RNA-Seq Kit: Strand-Specific Library Construction for Transcriptome Analysis on Illumina Platforms
The SMARTer Stranded RNA-Seq Kit provides a solution for generating libraries for Illumina® sequencing platforms that retain strand information with >99% accuracy. This kit generates robust data with input as low as 100 pg to 100 ng of RNA. The SMARTer Stranded RNA-Seq Kit uses a random priming method that is applicable to both polyA RNA and rRNA-removed total RNA. The kit can be used with both mammalian RNA and bacterial samples. The core SMART technology has been modified in this kit to generate complete transcriptome coverage of both coding and non-coding RNA while incorporating indexes and adaptors in the workflow, which eliminates the need for a downstream library preparation kit. You can generate indexed libraries that are ready for Illumina sequencing in less than 4 hours using the SMARTer Stranded RNA-Seq kit protocol.
Like other SMARTer kits for Next-Gen Sequencing (NGS), the SMARTer Stranded RNA-Seq Kit is a complete kit that includes all required reagents, including SMARTScribe Reverse Transcriptase and a new SeqAmp PCR polymerase that has been well-tested and verified to support the product's stellar performance.