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Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Single_cell_RNA_Seq_Kits_for_mRNA_seq >  Single_Cell_RNA_Seq_v3

SMARTer Ultra Low Input RNA Kit for Sequencing - v3—cDNA Synthesis for Single-Cell Transcriptome Analysis

In developing the innovative, new SMARTer Ultra Low Input RNA Kit for Sequencing - v3, we took the opportunity to review both our internal research over the past year and data generated in external laboratories in order to create a new system for ultra-low input transcriptome analysis (RNA-seq). This new kit builds on SMART (Switching Mechanism at 5’ End of RNA Template) technology, that Clontech has developed and provided for almost 20 years, and offers significant new improvements. The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 is designed to produce cDNA libraries for RNA-seq directly from single cells or very low-input total RNA. It generates full-length cDNA libraries from as few as 1–1,000 cells or as little as 10 pg–10 ng of total RNA. Compared to previous SMARTer Ultra Low kits that incorporated the SMART-Seq™ method, the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 identifies a greater number of genes, has higher transcript mapping, and has higher exon coverage. This kit is compatible with library preparation kits and sequencing from many suppliers, including Illumina and Ion Torrent.

The core SMART technology in this kit is an immensely powerful tool for studying the full transcriptome, including splice junctions and alternative splicing events in cells, and routinely provides full-length gene coverage. Oligo(dT) priming is used to specifically amplify mRNA from either high-quality total RNA (RIN >8) or intact cells.

Information about prior SMARTer Ultra Low RNA kits can be found here.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Sample prep made easy—The single-tube protocol works directly on whole cells and preserves sample integrity
  • Unparalleled sensitivity—Start with as little as 1 cell or 10 pg of total RNA (input range: 1–1,000 cells or 10 pg–10 ng of total RNA)
  • Improved cDNA amplification—The SeqAmp DNA Polymerase included in the kit better amplifies high GC-content genes
  • Suitable for multiple sequencing platforms—cDNA libraries are compatible with either Illumina® or Ion Torrent NGS platforms
  • High-quality RNA-seq data—Get a higher number of genes identified, full length gene analysis, typically less than 2% rRNA reads, and improved representation of GC-rich genes

Interested in more data and FAQs about this product? Visit the NGS Resource Portal

Applications

  • cDNA synthesis and library generation from small quantities of cells or total RNA for transcriptome sequencing
  • cDNA output from this kit can be used with either our Low Input Library Prep Kit or Nextera® sample preparation kits for Illumina sequencing, or with the Ion Xpress Plus Fragment Library Kit for Ion Torrent sequencing

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
634848 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 12 Rxns $1,482.00 License Statements
634849 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 24 Rxns $2,594.00 License Statements
634850 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 48 Rxns $4,152.00 License Statements
634851 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 96 Rxns $6,660.00 License Statements
634852 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 192 Rxns $10,560.00 License Statements
634853 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 480 Rxns $25,460.00 License Statements
 

 

Required Products
Cat. # Product Contents Size Price # of Units Select
638504 SeqAmp™ DNA Polymerase 50 Rxns $105.00
638509 SeqAmp™ DNA Polymerase 200 Rxns $295.00
 

 

Recommended Products
Cat. # Product Contents Size Price Units Select
634947 Low Input Library Prep Kit 12 Rxns $1,140.00
638325 DNA Standards for Library Quantification 50 Rxns $225.00
638324 Library Quantification Kit 500 Rxns $515.00
 
 
 

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