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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Single_cell_RNA_Seq_Kits_for_mRNA_seq >  Single_Cell_RNA_Seq_dT_Primed_High_Volume

SMARTer Ultra Low Input RNA Kit for Illumina Sequencing-HV—cDNA Synthesis for Transcriptome Analysis

The SMARTer Ultra Low RNA Kit for Illumina® Sequencing-HV provides a robust solution for generating libraries for transcriptome analysis (RNA-seq) directly from very low input of cells or total RNA. The kit is designed to work with as few as 1–1,000 cells or very low amounts of total RNA (10 pg–10 ng). The cDNA output from the kit can be used with either the Nextera® DNA sample preparation kit (using our modified protocol) or with our Low Input Library Prep Kit, to generate libraries that are compatible with Illumina's Genome Analyzer™, HiSeq®, or MiSeq® instruments.

This HV kit uses an oligo dT priming method, relies on using high quality RNA (RIN >8) or intact cells, and accommodates an input volume of 1–9 µl. It has been modified from our original SMARTer Ultra Low RNA Kit for Illumina Sequencing (maximum input volume = 1 µl) to accomodate input volumes of 1–9 µl, which may be helpful when working with either diluted samples or sorted cells in a larger volume.

The core SMART technology in this kit (also referred to as SMART-seq) guarantees full length gene coverage that outperforms existing methods in transcriptome analysis. SMART technology provides an immensely powerful tool for studying full gene coverage, splice junctions, and alternative splicing events in cells.

At-A-Glance Documents Images & Data Resources

Features

  • Sample prep made easy Use cells directly or total RNA.  Single-tube protocol works directly on cells to preserve sample integrity
  • Unparalleled sensitivity—Start with as little as 1 cell or 10 pg of total RNA (input range: 1–1,000 cells or 10 pg to 10 ng of total RNA)
  • Integration with Illumina sequencing—Compatible with Illumina's NGS platforms
  • Excellent RNA-seq data —Whole transcriptome information with full length gene analysis, minimal 5' or 3' bias, less than 2% rRNA reads, broadest coverage of exons, and ERCC correlation when compared to other technologies. 

Applications

  • cDNA synthesis and library generation from small quantities of cells or total RNA for transcriptome sequencing using Illumina's Genome Analyzer, HiSeq and MiSeq instruments
  • cDNA output from this kit can be used with either our Low Input Library Prep Kit or with the Nextera DNA sample preparation kit with our modified protocol

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Package Size Price License # of Units Select
634820 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 12 Rxns $1,560.00 License Statements
634823 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 24 Rxns $2,730.00 License Statements
634826 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 48 Rxns $4,370.00 License Statements
634828 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 96 Rxns $7,010.00 License Statements
634830 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 480 Rxns $26,800.00 License Statements
 

 

Required Products
Cat. # Product Package Size Price # of Units Select
639207 Advantage® 2 PCR Kit 30 Rxns $135.00
634947 Low Input Library Prep Kit 12 Rxns $1,140.00
 

 

 


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