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Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Single_cell_RNA_Seq_Kits_for_mRNA_seq >  Single_Cell_RNA_Seq_dT_Primed

SMARTer Ultra Low Input RNA Kit for Illumina Sequencing—cDNA Synthesis from Single Cells

The SMARTer Ultra Low RNA Kit for Illumina® Sequencing provides a simple and efficient solution for generating libraries for transcriptome analysis (mRNA-seq) from single cells, a few to 1,000 intact cells, or very low amounts of total RNA (10 pg–10 ng) that are compatible with Illumina's Genome Analyzer™, HiSeq®, or MiSeq® instruments. This kit uses oligo dT priming and allows cDNA synthesis directly from cells without any sample preparation or can use total RNA as the starting material. Clontech offers multiple kits for single cell transcriptome analysis. This kit requires an input volume of 1 µl. The SMARTer Ultra Low Input RNA for Illumina Sequencing - HV accommodates higher input volumes of 1–9 µl of cells or RNA.

The SMARTer Ultra Low RNA Kit for Illumina has also been reconfigured specifically for the single-cell mRNA-seq application on the Fluidigm C1 System. The core SMART technology in these kits is referred to as SMART-seq™ in the literature, and has shown to be an immensely powerful tool for studying full gene coverage, transcript counting, splice junctions and alternative splice variants in cells.

If the quality of your cells or RNA is lower than the recommended RIN value, we recommend the SMARTer Stranded RNA-seq Kit for generating strand specific libraries for Illumina platforms or SMARTer Universal Low Input RNA Kit.

At-A-Glance Documents Images & Data Resources

Features

  • Single-cell sensitivity—Start with as little as 10 pg of total RNA (input range 10 pg to 10 ng)
  • Works directly on cellsSingle-tube protocol that eliminates sample prep by working directly on cells
  • RNA-seq data quality—High mapping; high gene counts; full length gene analysis with no 5' or 3' bias
  • Integration with Illumina sequencing—Compatible with Illumina's NGS platforms using either Nextera reagents or our Low Input Library Prep kit.

Applications

Sample preparation from extremely small samples for transcriptome sequencing using Illumina's Genome Analyzer, HiSeq and MiSeq instruments.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
634936 SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing 100 Rxns $8,100.00 License Statements
 

 

Required Products
New Cat. # Product Package Size Price # of Units Select
  639207 Advantage® 2 PCR Kit 30 Rxns $135.00
634947 Low Input Library Prep Kit 12 Rxns $1,140.00
 

 

 


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