SMARTer Ultra Low Input RNA Kit for Illumina Sequencing—cDNA Synthesis from Single Cells
The SMARTer Ultra Low RNA Kit for Illumina® Sequencing provides a simple and efficient solution for generating libraries for transcriptome analysis (mRNA-seq) from single cells, a few to 1,000 intact cells, or very low amounts of total RNA (10 pg–10 ng) that are compatible with Illumina's Genome Analyzer™, HiSeq®, or MiSeq® instruments. This kit uses oligo dT priming and allows cDNA synthesis directly from cells without any sample preparation or can use total RNA as the starting material. Clontech offers multiple kits for single cell transcriptome analysis. This kit requires an input volume of 1 µl. The SMARTer Ultra Low Input RNA for Illumina Sequencing - HV accommodates higher input volumes of 1–9 µl of cells or RNA.
The SMARTer Ultra Low RNA Kit for Illumina has also been reconfigured specifically for the single-cell mRNA-seq application on the Fluidigm C1 System. The core SMART technology in these kits is referred to as SMART-seq™ in the literature, and has shown to be an immensely powerful tool for studying full gene coverage, transcript counting, splice junctions and alternative splice variants in cells.