Single-cell transcriptomics is important in understanding how gene expression of individual cells varies within cell populations. Takara Clontech has developed an end-capture method that focuses the majority of the sequencing data on the 3’ ends of mRNA transcripts from single cells. This enables you to collect high quality data with fewer sequencing reads. This kit incorporates features from the industry-leading SMART-Seq v4 kit, including SMART (Switching Mechanism at 5’ End of RNA Template) and locked nucleic acid (LNA) technology.
In addition to focusing reads on the 3’ end of mRNA transcripts, this kit also includes in-line indexes that serve as cell barcodes on Read 2 that enables pooling of up to 12 samples per each Illumina index pair, resulting in up to 1,152 samples per sequencing lane. This pooling further reduces the time and costs of discovery. This kit takes a convenient input volume of 1–10.5 µl and relies on the Illumina Nextera® XT DNA Library Preparation Kit. This kit has been designed and validated to prepare cDNA samples and subsequent sequencing libraries for the following Illumina sequencing platforms: HiSeq®, NextSeq®, MiSeq®, and MiniSeq™.