Clontech offers multiple generations of single-cell transcriptome analysis kits. The core SMART (Switching Mechanism at 5’ End of RNA Template) technology in these legacy kits is an immensely powerful tool for transcriptome and full-length transcript analyses, including investigations in splice junctions and alternative splice variants in cells. These kits use oligo(dT) priming and allow for cDNA synthesis directly from whole cells, without any additional sample preparation, or from total RNA of high integrity (RIN 8–10). cDNA libraries can be simply and efficiently generated for transcriptome analysis (mRNA-seq) from single cells, a few to 1,000 intact cells, or 10 pg–10 ng of total RNA.
All legacy SMARTer Ultra Low kits generate libraries that are compatible with Illumina® instruments. The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 has also been tested with Ion Torrent sequencing library preparation kits.
We recommend completing ongoing experiments with the same SMARTer Ultra Low legacy kit that you started with. For new experiments using the most advanced cDNA synthesis from single cells, we recommend the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing.