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Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Single_cell_RNA_Seq_Kits_for_mRNA_seq >  Legacy_Single_Cell_RNA_Seq

Legacy Single-Cell RNA-Seq Kits

Clontech offers multiple generations of single-cell transcriptome analysis kits. The core SMART (Switching Mechanism at 5’ End of RNA Template) technology in these legacy kits is an immensely powerful tool for transcriptome and full-length transcript analyses, including investigations in splice junctions and alternative splice variants in cells. These kits use oligo(dT) priming and allow for cDNA synthesis directly from whole cells, without any additional sample preparation, or from total RNA of high integrity (RIN 8–10). cDNA libraries can be simply and efficiently generated for transcriptome analysis (mRNA-seq) from single cells, a few to 1,000 intact cells, or 10 pg–10 ng of total RNA.

All legacy SMARTer Ultra Low kits generate libraries that are compatible with Illumina® instruments. The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 has also been tested with Ion Torrent sequencing library preparation kits.

We recommend completing ongoing experiments with the same SMARTer Ultra Low legacy kit that you started with. For new experiments using the most advanced cDNA synthesis from single cells, we recommend the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Sample prep made easy—Work directly on cells, minimize the chances of contamination, and simplify the workflow
  • RNA-seq data quality—A high percentage of reads map to the genome; high numbers of genes identified; full-length gene analysis with minimal 5' or 3' bias
  • Ultra-low input—Start with as little as 1 cell or 10 pg of total RNA (input range: 1–1,000 cells or 10 pg–10 ng of total RNA)

Interested in more data and FAQs about this product? Visit the NGS Resource Portal

After October, 2015, only the 48-, 96-, and 100-reaction kits will be available. Smaller and larger reaction sizes will no longer be available.

Applications

  • cDNA synthesis and library generation from small quantities of cells or total RNA for transcriptome sequencing.
  • cDNA output from these kits can be used with either our Low Input Library Prep Kit or with the Nextera® XT library prep kits. cDNA from the SMARTer Ultra Low Input RNA Kit for Sequencing -  v3 is also compatible with the Ion Xpress Plus Fragment Library Kit for Ion Torrent sequencing.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
634848 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 12 Rxns $1,482.00 License Statements
634851 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 96 Rxns $6,660.00 License Statements
634852 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 192 Rxns $10,560.00 License Statements
634853 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 480 Rxns $25,460.00 License Statements
634849 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 24 Rxns $2,594.00 License Statements
634850 SMARTer® Ultra™ Low Input RNA Kit for Sequencing - v3 48 Rxns $4,152.00 License Statements
634820 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 12 Rxns $1,560.00 License Statements
634823 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 24 Rxns $2,730.00 License Statements
634826 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 48 Rxns $4,370.00 License Statements
634828 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 96 Rxns $7,010.00 License Statements
634830 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 480 Rxns $26,800.00 License Statements
634936 SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing 100 Rxns $8,100.00 License Statements
 

 

Required Products
Cat. # Product Contents Size Price # of Units Select
639207 Advantage® 2 PCR Kit 30 Rxns $135.00
638504 SeqAmp™ DNA Polymerase 50 Rxns $105.00
638509 SeqAmp™ DNA Polymerase 200 Rxns $295.00
 

 

Recommended Products
Cat. # Product Contents Size Price Units Select
634947 Low Input Library Prep Kit 12 Rxns $1,140.00
638325 DNA Standards for Library Quantification 50 Rxns $225.00
638324 Library Quantification Kit 500 Rxns $515.00
 
 
 

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