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cDNA Synthesis & Library Construction

Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Single_cell_RNA_Seq_Kits_for_mRNA_seq >  Automated_mRNA-Seq_Library_Prep

SMART-Seq v4 Reagent Kit for the SMARTer Apollo System

The SMART-Seq v4 Reagent Kit for the SMARTer Apollo System is designed to generate high-quality, full-length cDNA directly from 1–1,000 cells or 10 pg–10 ng of total RNA using a high-throughput workflow that enables processing of up to 96 samples in a single working day. With this kit, we have reformatted our best-in-class method for ultra-low-input RNA-seq library prep for automation on the SMARTer Apollo platform, providing a walkway solution that performs comparably to manual workflows.

cDNA produced with this kit can be processed into NGS libraries suitable for analysis on Illumina® or Ion Torrent platforms. The kit incorporates the same technologies and chemistries associated with our other SMART-Seq v4 products, including oligo(dT)-priming, SMART template-switching technology, and locked nucleic acids (LNA), ensuring the production of cDNA libraries that provide full-length sequence information, accurate representation of GC-rich transcripts, and industry-leading sensitivity.

  At-A-Glance   Documents


  • Industry-leading performance—obtain an accurate representation of rare and GC-rich transcripts
  • Flexible inputs—generate high-quality cDNA libraries from 1–1,000 cells or 10 pg–10 ng of total RNA
  • Walkaway automation—minimize hands-on time and sample-handling errors using SMARTer Apollo scripts developed specifically for this protocol
  • High-throughput sample preparation—obtain purified cDNA from up to 96 samples in a single working day


  • High-throughput cDNA synthesis from low-input samples consisting of 1–1,000 cells or 10 pg–10 ng of total RNA
  • mRNA-seq on Illumina or Ion Torrent platforms

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

New Cat. # Product Contents Size Price License Units Select
640170 SMART-Seq® v4 Reagent Kit for the SMARTer™ Apollo™ System 96 Rxns $5,964.00 License Statements



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