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Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Ribosomal_RNA_Removal

RiboGone - Mammalian—Low Input Ribosomal RNA Removal Kit for Human, Mouse and Rat Samples

The RiboGone - Mammalian kit specifically removes ribosomal RNA (rRNA) and mitochondrial RNA (mtRNA) sequences from human, mouse or rat total RNA samples. The RiboGone technology is based on hybridization and RNase H digestion that specifically depletes 5S, 5.8S, 18S, and 28S nuclear rRNA sequences as well as 12S mtRNA sequences. The kit is designed to work with any quality starting RNA, including degraded material like FFPE samples. The RiboGone - Mammalian kit is highly suited for sample preparations prior to random-primed cDNA synthesis and RNA-seq or transcriptome analysis applications. Following the use of RiboGone - Mammalian, and the SMARTer Stranded RNA-Seq Kit or the SMARTer Universal Low Input RNA Library Prep Kit, rRNA reads have been recorded to be between 1-5% of total reads and lower.
  At-A-Glance   Documents   Images & Data   Resources

Features

  • Effective rRNA removal method for human, mouse, and rat total RNA samples—depletes ≥95% of ribosomal RNA sequences
  • Suitable for any quality RNA, including FFPE samples
  • Low input working range: 10–100 ng starting material
  • Works seamlessly with downstream random-primed cDNA synthesis kits like the Stranded RNA-Seq Kit or the SMARTer Universal Low Input RNA Library Prep Kit

Interested in more data and FAQs about this product? Visit the NGS Resource Portal

Applications

  • Removal of rRNA from small total RNA samples prior to random-primed cDNA synthesis


Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
 
 
Products
Cat. # Product Contents Size Price License Units Select
634846 RiboGone™ - Mammalian 6 Rxns $480.00 License Statements
634847 RiboGone™ - Mammalian 24 Rxns $1,680.00 License Statements
 

 

Required Products
Cat. # Product Contents Size Price # of Units Select
634938 SMARTer® Universal Low Input RNA Kit for Sequencing 10 Rxns $1,050.00
634940 SMARTer® Universal Low Input RNA Kit for Sequencing 25 Rxns $2,250.00
634945 SMARTer® Universal Low Input RNA Library Prep Kit 10 Rxns $1,800.00
634946 SMARTer® Universal Low Input RNA Library Prep Kit 25 Rxns $4,000.00
634836 SMARTer® Stranded RNA-Seq Kit 12 Rxns $960.00
634837 SMARTer® Stranded RNA-Seq Kit 24 Rxns $1,728.00
634838 SMARTer® Stranded RNA-Seq Kit 48 Rxns $2,920.00
634839 SMARTer® Stranded RNA-Seq Kit 96 Rxns $3,760.00
 

 

Recommended Products
Cat. # Product Contents Size Price Units Select
635011 SMARTer-Seq™ Magnetic Separator - PCR Strip Each $199.00
 
 
 

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