RiboGone - Mammalian—Low Input Ribosomal RNA Removal Kit for Human, Mouse and Rat Samples
The RiboGone - Mammalian kit specifically removes ribosomal RNA (rRNA) and mitochondrial RNA (mtRNA) sequences from human, mouse or rat total RNA samples. The RiboGone technology is based on hybridization and RNase H digestion that specifically depletes 5S, 5.8S, 18S, and 28S nuclear rRNA sequences as well as 12S mtRNA sequences. The kit is designed to work with any quality starting RNA, including degraded material like FFPE samples. The RiboGone - Mammalian kit is highly suited for sample preparations prior to random-primed cDNA synthesis and RNA-seq or transcriptome analysis applications. Following the use of RiboGone - Mammalian, and the SMARTer Stranded RNA-Seq Kit or the SMARTer Universal Low Input RNA Library Prep Kit, rRNA reads have been recorded to be between 1-5% of total reads and lower.