Clontech
login | register | order now | view cart | feedback
Products
cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Mouse_BCR_Profiling

SMARTer Mouse BCR IgG H/K/L Profiling Kit—Capturing Complete V(D)J Variable Regions of BCR Transcripts

B cells are essential parts of the adaptive immune response, expressing B-cell receptors (BCRs) on their surface which allow the recognition of unique molecular patterns in pathogens. Understanding the profiles of BCRs, (i.e., the diversity of receptors and the clonotypes, defined by expression of specific H, K, and L gene segments) can not only aid in gaining insights into the adaptive immune response in healthy individuals but also those with a wide range of diseases. Accurate determination of the clonotypes and isotypes expressed by the immune system will aid in a complete picture of the B-cell repertoire.

The new SMARTer Mouse BCR IgG H/K/L Profiling Kit pairs 5’ RACE with NGS technology to provide a sensitive, accurate, and optimized approach to BCR profiling. While systems that use multiplex PCR allow for the amplification of multiple BCR genes in one reaction, it may prove challenging with regard to sensitivity,specificity, and biases in the amplification of certain sequences, all of which can lead to difficulties in isotype identification. The 5’-RACE method reduces variability and allows for priming from the constant region of BCR heavy or light chains. This kit combines these benefits with gene-specific amplification to capture complete V(D)J variable regions of BCR transcripts and provide a highly sensitive and reproducible method for profiling B-cell repertoires. Downstream sequencing of H/K/L chains allows for accurate identification of top clonotypes and reliable assignment of isotype in a majority of cases.

  At-A-Glance   Documents   Images & Data

Features

  • Input:10 ng–3 µg of total RNA from spleen, lymph node, PBMCs, and hybridomas
  • Sensitive and specific clonotype detection with optimized cDNA library generation
  • Accurate amplification of mouse IgG subclasses and identification via sequencing in a majority of cases
  • Optimized PCR pooling strategy for highly sensitive detection of different chains from the same sample
  • Flexible pooling strategies accommodate different experimental requirements regarding alignment and identification of primary chain sequences

Applications

  • Mouse BCR repertoire analysis (heavy and light [kappa and lambda] chains)

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

 
 
Products
New Cat. # Product Contents Size Price Units Select
634422 SMARTer® Mouse BCR IgG H/K/L Profiling Kit 12 Rxns $900.00
634423 SMARTer® Mouse BCR IgG H/K/L Profiling Kit 48 Rxns $3,072.00
634424 SMARTer® Mouse BCR IgG H/K/L Profiling Kit 96 Rxns $4,915.00
 

 

Recommended Products
Cat. # Product Contents Size Price License Units Select
635011 SMARTer-Seq™ Magnetic Separator - PCR Strip Each $199.00  
634402 SMARTer® Mouse TCR a/b Profiling Kit 12 Rxns $960.00 License Statements
634403 SMARTer® Mouse TCR a/b Profiling Kit 48 Rxns $3,072.00 License Statements
634404 SMARTer® Mouse TCR a/b Profiling Kit 96 Rxns $4,915.00 License Statements
 
 
 

Takara Bio USA is a Takara Bio Company © 2017 Privacy Policy | Terms & Conditions | Terms of Use