EpiXplore Meth-Seq DNA Enrichment Kit—Next-Gen Sequencing of Methylated DNA for Epigenetics
The EpiXplore Meth-Seq DNA Enrichment Kit is a complete system for separating methylated and unmethylated fractions of sheared genomic DNA, and generating sequencing-ready Illumina® libraries from the resulting enriched DNA fractions. The enrichment protocol utilizes the his-tagged MBD2 protein currently used in the EpiXplore Methylated DNA Enrichment Kit in conjunction with Capturem Nanoprep Columns to separate methylated DNA from unmethylated DNA in ~2 hours. Illumina-compatible sequencing libraries are then generated from the methylated (or unmethylated, if desired) DNA fractions using DNA SMART technology.
For library prep, the DNA SMART method has been adapted for the addition of Illumina-specific sequencing adapters to the ends of DNA fragments, in a ligation-independent manner. This highly sensitive technology generates robust sequencing libraries from low-input DNA samples, with minimal handling, in about four hours.