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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Library_Construction_Kit_for_Illumina_Sequencing

Low Input DNA Library Prep Kits for Illumina

The Low Input Library Prep Kit v2 and Low Input Library Prep Kit HT use a highly sensitive, simple, fast, and robust method to generate multiplexed libraries suitable for next-generation sequencing on Illumina® systems from small amounts of DNA. This method is exceptionally suited for cDNA produced using the SMARTer Universal Low Input RNA Kit for Sequencing, the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing, and the SMARTer Ultra Low Input legacy kits. These multiplexed libraries are suitable templates for cluster generation and high-throughput sequencing.

  • The Low Input Library Prep Kit v2 (Cat. # 634899) produces Illumina-compatible libraries from a low amount of dsDNA. The kit comes with 12 indexing reagents pre-dispensed into tubes containing 8-nucleotide Sanger indexes that share the same sequences in the first 6 bases as the Illumina TruSeq LT indexes AD001 through AD012.
  • The Low Input Library Prep Kit HT (Cat. # 634900) has higher multiplexing capability and an optimized protocol for converting up to 96 low-input samples of fragmented, double-stranded DNA (dsDNA) into a sequencing-ready library for Illumina platforms. It comes with 96 dual-read, Illumina-compatible indexes pre-dispensed and sealed in microplates. The indexing reagent is a unique combination of Illumina's 8-nucleotide TruSeq HT i5 and i7 index sequences.

  At-A-Glance   Documents   Images & Data

Features

NOTE: The Low Input Library Prep Kit (Cat. # 634947) will be discontinued at the end of 2016. However, you can continue to purchase the same technology with multiplexing capability: the Low Input Library Prep Kits v2 (Cat. # 634899) and HT (Cat. # 634900).
  • Converts fragmented double-stranded DNA (dsDNA) into sequencing-ready libraries for Illumina platforms
  • Low-input compatible, down to 50 pg of dsDNA
  • Single-tube, three-step protocol eliminates intermediate purification steps and takes just 2 hours

Applications

  • Singleplex or multiplex next-generation sequencing on Illumina systems
  • Single-read or paired-end sequencing

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
634947 Low Input Library Prep Kit 12 Rxns $1,140.00 License Statements
634899 Low Input Library Prep Kit v2 12 Rxns $900.00 License Statements
634900 Low Input Library Prep Kit HT 96 Rxns $5,280.00 License Statements
 

 

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