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Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  DNA-Seq_Kits >  Single-Cell_DNA_Library_Prep_for_Illumina

SMARTer PicoPLEX DNA-seq Kit—Single-Cell Library Preparation for Illumina NGS Platforms

SMARTer PicoPLEX technology, used to detect chromosomal copy number variation in single cells on arrays, is now available in a single-tube format for use on Illumina NGS platforms. SMARTer PicoPLEX WGA can be used in a wide range of applications and has long been used by IVF clinics worldwide for amplification of DNA from embryonic cells prior to their preimplantation genetic screening, and for detecting chromosomal aneuploidies and copy number variations.

The SMARTer PicoPLEX DNA-seq Kit streamlines library preparation; the entire process is performed in a single tube or well from as little as 1–10 cells (15–50 pg gDNA), thus reducing error and contamination, speeding up time to results, and reducing costs. The kit contains 48 reactions and includes everything necessary to convert 48 individual cells or DNA (6 pg to 60 pg) to barcoded NGS libraries. Barcoding oligonucleotides are provided in a single-use microwell plate.

The recommended sequencing platforms for SMARTer PicoPLEX libraries are the Illumina MiSeq® System and HiSeq® 2000/2500. For use on the Illumina NextSeq® sequencer, please contact techUS@takarabio.com prior to library preparation.

  At-A-Glance   Documents

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Features

  • Reduce ambiguity: highly reproducible CNV and aneuploidy detection
  • Reduce workflow: from a single cell to a sequencing-ready library in three steps
  • Reduce cost: a single kit contains everything needed to prepare a sequencing-ready library
  • Reduce contamination and error: library prep in a single tube or well; no transfers necessary
  • Reduce time to results: Illumina NGS libraries prepared in less than three hours

Applications

Library preparation from the following cell types:

  • Reproductive health: embryonic cells to be used for subsequent preimplantation genetic screening, and circulating fetal cells for cell-based, noninvasive prenatal screening
  • Developmental biology: embryonic cells, stem cells, and rare cells
  • Cancer research: circulating tumor cells, cells or DNA from laser capture microdissection (LCM), and cells from fine needle aspirate (FNA)
  • Metagenomics and microbiology: microbial cells

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
R300381 PicoPLEX® DNA-seq Kit 48 Rxns $1,824.00 License Statements
 

 

 
 

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