SMARTer PicoPLEX DNA-seq Kit—Single-Cell Library Preparation for Illumina NGS Platforms
SMARTer PicoPLEX technology, used to detect chromosomal copy number variation in single cells on arrays, is now available in a single-tube format for use on Illumina NGS platforms. SMARTer PicoPLEX WGA can be used in a wide range of applications and has long been used by IVF clinics worldwide for amplification of DNA from embryonic cells prior to their preimplantation genetic screening, and for detecting chromosomal aneuploidies and copy number variations.
The SMARTer PicoPLEX DNA-seq Kit streamlines library preparation; the entire process is performed in a single tube or well from as little as 1–10 cells (15–50 pg gDNA), thus reducing error and contamination, speeding up time to results, and reducing costs. The kit contains 48 reactions and includes everything necessary to convert 48 individual cells or DNA (6 pg to 60 pg) to barcoded NGS libraries. Barcoding oligonucleotides are provided in a single-use microwell plate.
The recommended sequencing platforms for SMARTer PicoPLEX libraries are the Illumina MiSeq® System and HiSeq® 2000/2500. For use on the Illumina NextSeq® sequencer, please contact techUS@takarabio.com prior to library preparation.