SMARTer PicoPLEX technology, used to detect chromosomal copy number variation in single cells on arrays, is now available in a single-tube format for use on Illumina NGS platforms. SMARTer PicoPLEX WGA can be used in a wide range of applications and has long been used by IVF clinics worldwide for amplification of DNA from embryonic cells prior to their preimplantation genetic screening, and for detecting chromosomal aneuploidies and copy number variations.
The SMARTer PicoPLEX DNA-seq Kit streamlines library preparation; the entire process is performed in a single tube or well from as little as 1–10 cells (or <15–60 pg purified gDNA), thus reducing error and contamination, speeding up time to results, and reducing costs. The kit contains 48 reactions and includes everything necessary to convert 48 individual cells or DNA samples to barcoded NGS libraries. Barcoding oligonucleotides are provided in a single-use microwell plate.
The recommended sequencing platforms for SMARTer PicoPLEX libraries are the Illumina MiSeq® System and HiSeq® 2000/2500. For use on the Illumina NextSeq® sequencer, please contact techUS@takarabio.com prior to library preparation.
SMARTer DNA HT dual index kits
SMARTer DNA HT dual index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. # R400660−R400663; coming soon); or in a set of 24 individual tubes, each containing a different dual index combination (Cat. # R400664; available now). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.