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Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  DNA-Seq_Kits >  Single-Cell_DNA_Library_Prep_for_Illumina

SMARTer PicoPLEX DNA-seq Kit—Single-Cell Library Preparation for Illumina NGS Platforms

SMARTer PicoPLEX technology, used to detect chromosomal copy number variation in single cells on arrays, is now available in a single-tube format for use on Illumina NGS platforms. SMARTer PicoPLEX WGA can be used in a wide range of applications and has long been used by IVF clinics worldwide for amplification of DNA from embryonic cells prior to their preimplantation genetic screening, and for detecting chromosomal aneuploidies and copy number variations.

The SMARTer PicoPLEX DNA-seq Kit streamlines library preparation; the entire process is performed in a single tube or well from as little as 1–10 cells (or <15–60 pg purified gDNA), thus reducing error and contamination, speeding up time to results, and reducing costs. The kit contains 48 reactions and includes everything necessary to convert 48 individual cells or DNA samples to barcoded NGS libraries. Barcoding oligonucleotides are provided in a single-use microwell plate.

The recommended sequencing platforms for SMARTer PicoPLEX libraries are the Illumina MiSeq® System and HiSeq® 2000/2500. For use on the Illumina NextSeq® sequencer, please contact techUS@takarabio.com prior to library preparation.

SMARTer DNA HT dual index kits

SMARTer DNA HT dual index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. # R400660−R400663; coming soon); or in a set of 24 individual tubes, each containing a different dual index combination (Cat. # R400664; available now). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.

SMARTer DNA unique dual index kits

SMARTer DNA unique dual index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the “IDT for Illumina UD” index sequences and offer a total of 96 dual indexes for multiplexing of up to 96 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 24 individual tubes, each of which contains one-fourth of the possible dual index combinations (Cat. Nos. R400665−R400668). Each dual index tube (24U Sets A–D) contains sufficient volume for up to two uses.

  At-A-Glance   Documents

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Features

  • Reduce ambiguity: highly reproducible CNV and aneuploidy detection
  • Reduce workflow: from a single cell to a sequencing-ready library in three steps
  • Reduce cost: a single kit contains everything needed to prepare a sequencing-ready library
  • Reduce contamination and error: library prep in a single tube or well; no transfers necessary
  • Reduce time to results: Illumina NGS libraries prepared in less than three hours

Applications

Library preparation from the following cell types:

  • Reproductive health: embryonic cells to be used for subsequent preimplantation genetic screening, and circulating fetal cells for cell-based, noninvasive prenatal screening
  • Developmental biology: embryonic cells, stem cells, and rare cells
  • Cancer research: circulating tumor cells, cells or DNA from laser capture microdissection (LCM), and cells from fine needle aspirate (FNA)
  • Metagenomics and microbiology: microbial cells

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
  R300381 SMARTer® PicoPLEX® DNA-seq Kit 48 Rxns $1,824.00 License Statements
R400664 SMARTer® DNA HT Dual Index Kit - 24N 48 Rxns $328.00  
R400665 SMARTer® DNA Unique Dual Index Kit - 24U Set A 48 Rxns $312.00  
R400666 SMARTer® DNA Unique Dual Index Kit - 24U Set B 48 Rxns $312.00  
R400667 SMARTer® DNA Unique Dual Index Kit - 24U Set C 48 Rxns $312.00  
R400668 SMARTer® DNA Unique Dual Index Kit - 24U Set D 48 Rxns $312.00  
 

 

 
 

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