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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  DNA-Seq_Kits >  DNA_Library_Prep_for_Illumina

ThruPLEX DNA-seq Kit—General Purpose Library Preparation for Illumina NGS Platforms

ThruPLEX DNA-seq kits build on the innovative ThruPLEX chemistry to generate DNA libraries from as little as 50 pg of DNA and increase sensitivity while providing up to 96 indexes for multiplexing. These kits can be used with fragmented DNA from any sample source—genomic DNA, biofluids such as cell-free DNA, DNA from FFPE materials, and cDNA. The entire ThruPLEX DNA-seq kit workflow is performed in a single tube or well in about 2 hours and requires no purification steps or sample transfers. Kits contain 12, 48, or 96 single- or dual-read Illumina-compatible indexes either in tubes (6 or 12) or pre-dispensed and sealed in barcoded microplates. The ThruPLEX DNA-seq kits can be used in any DNA-seq application, RNA-seq, or ChIP-seq and offer robust target enrichment performance with all of the leading platforms.

SMARTer DNA HT dual index kits

SMARTer DNA HT dual index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. # R400660−R400663; coming soon); or in a set of 24 individual tubes, each containing a different dual index combination (Cat. # R400664; available now). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.

SMARTer DNA unique dual index kits

SMARTer DNA unique dual index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the “IDT for Illumina UD” index sequences and offer a total of 96 dual indexes for multiplexing of up to 96 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 24 individual tubes, each of which contains one-fourth of the possible dual index combinations (Cat. Nos. R400665−R400668). Each dual index tube (24U Sets A–D) contains sufficient volume for up to two uses.

  At-A-Glance   Documents

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Features

  • High performance from lowest input—50 pg to 50 ng
  • Complete package for creating libraries—optimized indexing and adapter reagents, enzymes, buffers, and nuclease-free water
  • Fast and simple workflow—three steps in a single tube or well in about 2 hours, no transfers necessary
  • Compatible with major target enrichment platforms—Agilent SureSelect, Roche Nimblegen SeqCap EZ, and IDT xGen Lockdown probes

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
  R400406 SMARTer® ThruPLEX® DNA-seq 48D Kit 48 Rxns $1,920.00 License Statements
  R400407 SMARTer® ThruPLEX® DNA-seq 96D Kit 96 Rxns $3,456.00 License Statements
  R400427 SMARTer® ThruPLEX® DNA-seq 48S Kit 48 Rxns $1,920.00 License Statements
  R400428 ThruPLEX® DNA-seq 12S (48) Kit 48 Rxns $1,920.00 License Statements
  R400523 SMARTer® ThruPLEX® DNA-seq 6S (12) Kit 12 Rxns $540.00 License Statements
R400664 SMARTer® DNA HT Dual Index Kit - 24N 48 Rxns $328.00  
R400665 SMARTer® DNA Unique Dual Index Kit - 24U Set A 48 Rxns $312.00  
R400666 SMARTer® DNA Unique Dual Index Kit - 24U Set B 48 Rxns $312.00  
R400667 SMARTer® DNA Unique Dual Index Kit - 24U Set C 48 Rxns $312.00  
R400668 SMARTer® DNA Unique Dual Index Kit - 24U Set D 48 Rxns $312.00  
 

 

 
 

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