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cDNA Synthesis & Library Construction

Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  DNA-Seq_Kits >  Cell-Free_DNA-Seq_for_Illumina

ThruPLEX Plasma-seq Kit—Cell-Free DNA Library Preparation for Illumina NGS Platforms

ThruPLEX Plasma-seq is powered by ThruPLEX chemistry to generate high-performance NGS libraries from cell-free DNA isolated from plasma.

The chemistry is optimized specifically for cell-free DNA to maximize the library complexity and to preserve the GC representation of the input DNA. Libraries can be used for CNV analysis, whole  genome sequencing applications, or enrichment using your custom panels or leading target enrichment platforms, such as Agilent SureSelect and Roche NimbleGen SeqCap EZ.

Libraries prepared with ThruPLEX Plasma-seq provide added sensitivity to better understand genetic variations found within the plasma samples. Plasma, along with other liquid biopsies, has gained significant interest in the research community as a valuable and easily available sample type. These communities include translational research in oncology and developmental research involving the fetal fraction derived from maternal plasma.

SMARTer DNA HT dual index kits

SMARTer DNA HT dual index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. # R400660−R400663; coming soon); or in a set of 24 individual tubes, each containing a different dual index combination (Cat. # R400664; available now). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.

  At-A-Glance   Documents

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  • Designed for cell-free DNA—newly formulated repair and ligation reagents with optimized protocols
  • High-performance NGS libraries—high diversity with broad and reproducible GC coverage
  • Variable sample input—<1 ng to 30 ng of cell-free DNA
  • Fast and simple workflow—3 steps in a single tube or well in 2 hours, with no purification or sample transfer steps
  • Research applications—libraries from samples where results require high sensitivity including liquid biopsies, ctDNA, and targeted sequencing
  • Automation friendly—Beckman FXP Workstation

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

New Cat. # Product Contents Size Price License Units Select
  R400490 ThruPLEX® Plasma-seq 12S Kit 12 Rxns $624.00 License Statements
  R400491 ThruPLEX® Plasma-seq 48S Kit 48 Rxns $2,160.00 License Statements
  R400492 ThruPLEX® Plasma-seq 96D Kit 96 Rxns $3,888.00 License Statements
R400664 SMARTer® DNA HT Dual Index Kit - 24N 48 Rxns $328.00  



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