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cDNA Synthesis & Library Construction

Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  DNA-Seq_Kits >  Cell-Free_DNA-Seq_for_Illumina

ThruPLEX Plasma-seq Kit—Cell-Free DNA Library Preparation for Illumina NGS Platforms

ThruPLEX Plasma-seq is powered by ThruPLEX chemistry to generate high-performance NGS libraries from cell-free DNA isolated from plasma.

The chemistry is optimized specifically for cell-free DNA to maximize the library complexity and to preserve the GC representation of the input DNA. Libraries can be used for CNV analysis, whole  genome sequencing applications, or enrichment using your custom panels or leading target enrichment platforms, such as Agilent SureSelect and Roche NimbleGen SeqCap EZ.

Libraries prepared with ThruPLEX Plasma-seq provide added sensitivity to better understand genetic variations found within the plasma samples. Plasma, along with other liquid biopsies, has gained significant interest in the research community as a valuable and easily available sample type. These communities include translational research in oncology and developmental research involving the fetal fraction derived from maternal plasma.

  At-A-Glance   Documents

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  • Designed for cell-free DNA—newly formulated repair and ligation reagents with optimized protocols
  • High-performance NGS libraries—high diversity with broad and reproducible GC coverage
  • Variable sample input—<1 ng to 30 ng of cell-free DNA
  • Fast and simple workflow—3 steps in a single tube or well in 2 hours, with no purification or sample transfer steps
  • Research applications—libraries from samples where results require high sensitivity including liquid biopsies, ctDNA, and targeted sequencing
  • Automation friendly—Beckman FXP Workstation

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Cat. # Product Contents Size Price License Units Select
R400490 ThruPLEX® Plasma-seq 12S Kit 12 Rxns $624.00 License Statements
R400491 ThruPLEX® Plasma-seq 48S Kit 48 Rxns $2,160.00 License Statements
R400492 ThruPLEX® Plasma-seq 96D Kit 96 Rxns $3,888.00 License Statements



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