ThruPLEX Plasma-seq is powered by ThruPLEX chemistry to generate high-performance NGS libraries from cell-free DNA isolated from plasma.
The chemistry is optimized specifically for cell-free DNA to maximize the library complexity and to preserve the GC representation of the input DNA. Libraries can be used for CNV analysis, whole genome sequencing applications, or enrichment using your custom panels or leading target enrichment platforms, such as Agilent SureSelect and Roche NimbleGen SeqCap EZ.
Libraries prepared with ThruPLEX Plasma-seq provide added sensitivity to better understand genetic variations found within the plasma samples. Plasma, along with other liquid biopsies, has gained significant interest in the research community as a valuable and easily available sample type. These communities include translational research in oncology and developmental research involving the fetal fraction derived from maternal plasma.
SMARTer DNA HT dual index kits
SMARTer DNA HT dual index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. # R400660−R400663; coming soon); or in a set of 24 individual tubes, each containing a different dual index combination (Cat. # R400664; available now). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.