The ChIP Elute Kit uses a fast and simple method to reverse cross-links, elute, and purify DNA captured by a chromatin immunoprecipitation (ChIP) experiment. This kit replaces long and tedious protocols for the final stages of ChIP experiments. Single-stranded DNA (ssDNA) can be recovered in approximately one hour (compared to traditional methods that may take up to overnight) and is directly compatible with the DNA SMART ChIP-Seq Kit.
The DNA SMART ChIP-Seq Kit adds Illumina®-specific sequencing adapters to the ends of DNA fragments in a ligation-independent manner. Generating libraries for ChIP sequencing (ChIP-seq) can be difficult due to the small amounts of DNA recovered from ChIP experiments, particularly when using antibodies against low-abundance transcription factors. The DNA SMART ChIP-Seq Kit can generate sequencing libraries for Illumina platforms from 100 pg–10 ng of input DNA without using ligation. Either ssDNA or double-stranded DNA (dsDNA) from ChIP experiments can be used directly with this kit, making it compatible with DNA recovered with the ChIP Elute Kit or by other methods. This highly sensitive kit generates robust sequencing libraries from low-input DNA samples, with minimal handling, in about four hours.
The DNA SMART ChIP-Seq Kit uses a novel version of Clontech’s proprietary SMART technology, adapted for use with DNA templates, to generate next-generation sequencing (NGS) libraries for Illumina platforms. The core template switching technology of the DNA SMART ChIP-Seq Kit enables ligation-free addition of Illumina sequencing adapters to low-input samples.