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cDNA Synthesis & Library Construction

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Selection Guide

SMARTer cDNA Synthesis Kits for Next-Gen Sequencing

SMARTer cDNA synthesis is the industry standard for experiments that require exceptional sensitivity and complete transcriptome coverage, because it synthesizes accurate, full-length cDNA directly from cells or total RNA. The same core technology is included in a variety of SMARTer kits, designed for use with sorted cells, LCM or FFPE samples, and total RNA. Kits are available for leading sequencing platforms and Fluidigm’s C1 system.

NGS Selection Guide

mRNA-Seq  (dT priming)
Total RNA-Seq  (N6 priming)*


With strand information

Without strand information


* Ribosomal RNA removal required

SAMPLE TYPE (Input amount)
Cells and high-quality RNA
(1–1,000 cells; 10 pg–10 ng RNA)
LCM samples, FFPE tissue,
prokaryotic samples,
or samples with degraded RNA
(2–100 ng total RNA)
1 SMARTer® Ultra™ Low RNA Kit for Sequencing - v3
2 SMARTer Stranded RNA-Seq Kit
3 SMARTer Universal Low Input RNA Kit for Sequencing
4 SMARTer Ultra Low RNA Kit for the Fluidigm C1 System
RG RiboGone™ - Mammalian (for Ribosomal RNA Removal)
orange dot Illumina platform
blue dot Fluidigm C1 cell capture platform
purple dot Compatible with Illumina
and Ion Torrent platforms
2 RG 3 RG"
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