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cDNA Synthesis & Library Construction


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Selection Guide

cDNA Synthesis and Library Preparation Kits for Next-Gen Sequencing

Highly sensitive, complete transcriptome coverage directly from cells or total RNA. These cDNA synthesis and library preparation kits for RNA-seq are designed for use with sorted cells, LCM or FFPE samples, and isolated total RNA. Kits are available for leading sequencing platforms and Fluidigm's C1 system.

If you are interested in next-generation sequencing from DNA samples see our DNA SMART ChIP-Seq Kit.

RNA-Seq Selection Guide

APPLICATION
mRNA-Seq  (dT priming)
Total RNA-Seq  (N6 priming)*

 

With strand information

Without strand information

 

* Ribosomal RNA removal required

SAMPLE TYPE (Input amount)
     
Cells and high-quality total RNA
(1–1,000 cells; 10 pg–10 ng total RNA)
1
4
LCM samples, FFPE tissue,
prokaryotic samples,
or samples with degraded RNA
(2–100 ng total RNA)
2+RG
3+RG
High input total RNA samples, any quality
(100 ng–1 µg total RNA)
5
1 SMARTer Ultra Low Input RNA Kit for Sequencing - v3
2 SMARTer Stranded RNA-Seq Kit
3 SMARTer Universal Low Input RNA Kit for Sequencing
4 SMARTer Ultra Low RNA Kit for the Fluidigm C1 System
5 SMARTer Stranded Total RNA Sample Kit - HI Mammalian
RG RiboGone - Mammalian (for ribosomal RNA removal)
INSTRUMENT COMPATIBILITY
orange dot Illumina® platform
blue dot Fluidigm C1 cell capture platform
purple dot Compatible with Illumina
and Ion Torrent platforms
2 RG 3 RG"
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