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NGS Webinars

NGS WEBINAR SERIES

SMARTer solutions for next-generation sequencing

  • Sensitive and robust tools that provide comprehensive sample coverage for NGS experiments
  • High-performance kits for challenging applications such as single-cell mRNA-seq, total RNA-seq (including FFPE samples), and analysis of cell-free DNA obtained from biofluids
  • New products for immune profiling and epigenetics research
Select your application to watch relevant webinars:

Mutational and Copy Number Analysis of Cell-Free DNAs in Human Cancers

In this webinar, Dr. Liang Wang (Professor of Pathology and Molecular Genetics, Medical College of Wisconsin) shares insights from his recent work using ThruPLEX technology for next-generation sequencing of cell-free DNA to identify biomarkers for prostate, lung, and colon cancers.

Differential Gene Expression Analysis from Single Cells by 3' End Capture

In this webinar, Dr. Magnolia Bostick (Clontech Laboratories, Inc.) presents data generated with the SMART-Seq v4 3' DE Kit. This kit leverages our industry-leading method for single-cell RNA-seq to generate high-quality Illumina-ready libraries that capture 3' ends of RNA transcripts and are ideally suited for quantifying differences in gene expression on a genome-wide scale.

Single-Cell RNA-Seq Optimization: Working SMARTer with the Mantis

In this webinar sponsored by Formulatrix and Takara Clontech, three speakers discuss optimization of single-cell RNA-seq using SMART-Seq v4 chemistry on the Mantis.

  • Rahul Satija, Ph.D. (New York Genome Center & NYU): High-throughput automation of single-cell library preparation
  • Magnolia Bostick, Ph.D. (Clontech Laboratories, Inc.): Harnessing the power of SMART-Seq v4 in miniaturized reactions
  • Paul Ventura (Formulatrix, Inc.): Application of desktop microfluidic technologies in genomic sample prep workflows

Evaluating the SMART-Seq v4 method for mRNA-Seq with the Fluidigm C1 system

In this webinar about the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, IFCs, Dr. Charles Gawad (St. Jude Children’s Research Hospital) compares high-throughput single-cell mRNA-seq data generated with the Fluidigm C1 system using either the SMART-Seq v4 method or the original v1 kit.

New technologies for sensitive, low-input RNA-seq

In this webinar, Dr. Magnolia Bostick (Clontech Laboratories, Inc.) discusses the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing. This brand new kit produces high-quality data from a single, intact cell (or as little as 10 pg of input RNA). It incorporates technologies, like LNA, to generate RNA-seq libraries with higher sensitivity (identifying more transcripts) than any previous method.

SMARTer tools for single-cell RNA-seq

In this webinar, Dr. Rachel Fish (Clontech Laboratories, Inc.) presents data generated with the SMARTer Ultra Low Input RNA Kit for Sequencing - v3, which provides an improved method for cDNA synthesis for transcriptome analysis from ultra-low input RNA samples and intact cells.

Single-cell RNA-Seq with SMART technology

In this webinar, Dr. Andrew Farmer (Vice President of R&D, Clontech Laboritories, Inc.) explains how the SMARTer method of cDNA synthesis facilitates studies of transcriptome complexity from very small samples (1 to 1,000 cells, or 10 pg to 10 ng of total RNA).

A New Method for SMARTer Library Preparation from Challenging RNA Samples

In this webinar, Dr. Nathalie Bolduc (Takara Bio USA, Inc.) presents data generated with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, an updated version of our industry-leading solution for transcriptome analysis from challenging samples. This kit generates Illumina-ready sequencing libraries from 250 pg–10 ng mammalian total RNA inputs of any quality, including RNA obtained from FFPE and cell-free samples, and provides improved sequencing performance on all platforms.

Pushing the Limit: Transcriptome Analysis from Picogram Input Amounts of Total RNA

In this webinar, Dr. Nathalie Bolduc (Clontech Laboratories, Inc.) discusses the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. This new kit blends advanced technologies to enable ligation-free preparation of stranded Illumina-ready libraries from picogram input amounts of total RNA, including samples isolated from FFPE tissues.

SMARTer Methods for Total RNA-Seq

In this webinar, Dr. Magnolia Bostick (Clontech Laboratories, Inc.) discusses the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian. This kit works with 100 ng–1 µg total RNA samples (including RNA derived from FFPE tissues) and contains technology to remove rRNA and generate Illumina RNA-seq libraries in approximately 5 hours.

SMARTer tools for Ion Torrent sequencing

In this webinar, Dr. Magnolia Bostick (Clontech Laboratories, Inc.) discusses the SMARTer Universal Low Input RNA Kit for Sequencing and the RiboGone - Mammalian rRNA removal kit. These kits allow you to generate cDNA from low-input or degraded total RNA samples and sequence on either Illumina or Ion Torrent platforms.

SMARTer methods for strand-specific RNA-seq

In this webinar, Dr. Craig Betts (Clontech Laboratories, Inc.) discusses the SMARTer Stranded RNA-Seq Kit, which enables you to capture transcriptome complexity (including strand-of-origin information) from low-input samples.

SMARTer solutions for ribosomal RNA removal

In this webinar, Dr. Magnolia Bostick (Clontech Laboratories, Inc.) presents data showing how the RiboGone - Mammalian rRNA removal kit complements random-primed SMARTer cDNA synthesis from low-input RNA samples, including RNA isolated from FFPE tissues.

A SMARTer Approach to Small RNA Sequencing

In this webinar, Dr. Nathalie Bolduc (Clontech Laboratories, Inc.) presents data generated with the SMARTer smRNA-Seq Kit for Illumina. This kit generates high-quality, Illumina-ready sequencing libraries from 1 ng–2 µg of total RNA or enriched small RNA, and can be used to analyze the expression of diverse small RNA species, including miRNAs and piRNAs.

ChIP-Seq Library Preparation

In this webinar, Dr. Nathalie Bolduc (Clontech Laboratories, Inc.) discusses the DNA SMART ChIP-Seq Kit. This kit generates Illumina sequencing libraries, in a ligation-independent manner, from low-input single-stranded and double-stranded ChIP DNA.

A SMARTer approach to B-cell receptor repertoire analysis

In this webinar, Dr. Rachel Fish (Takara Bio USA, Inc.) discusses the key technical features and workflow advantages of the SMARTer Mouse BCR IgG H/K/L Profiling Kit and presents application data on the sensitive detection of full-length CDR3 sequences in V(D)J transcripts, clonotype identification in hybridomas, and the profiling of spleen-derived B-cell receptor (BCR) repertoires.

A SMARTer approach to mouse T-cell receptor repertoire analysis

In this webinar, Dr. Sarah Taylor (Takara Bio USA, Inc.) discusses application data from studies using the SMARTer Mouse TCR a/b Profiling Kit. This kit employs a 5' RACE-like approach to capture full-length V(D)J variable regions and generate Illumina-ready libraries for TCR alpha and/or beta chains.

A SMARTer approach to human T-cell receptor repertoire analysis

In this webinar, Dr. Sarah Taylor (Clontech Laboratories, Inc.) discusses the SMARTer Human TCR a/b Profiling Kit. This kit employs a 5' RACE-based approach to yield Illumina-compatible sequencing libraries from 10 ng–3 µg of human blood total RNA or from 50–10,000 purified T cells. It enables users to obtain full-length V(D)J sequence information for TCRα and/or TCRβ.

A SMARTer Approach to Targeted RNA Capture

In this webinar, Dr. Rachel Fish (Clontech Laboratories, Inc.) presents data generated with SMARTer Target RNA Capture for Illumina. This kit is a complete system designed to generate cDNA from enriched total RNA, allowing researchers to improve sequence coverage, identify rare gene fusions, and save costs with a targeted RNA-seq approach.

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