Open platform for single-cell analysis
High-throughput single-cell isolation, selection, and processing
- Be in control—selectively process only the cells of choice
- Be more confident in your data—reduce batch effects and the unnecessary analysis of empty or multi‑cell wells
Cell dispensing and visualization. After the ICELL8 MutliSample NanoDispenser (MSND) has dispensed cells into the 5,184-nanowell chip, every well is imaged. Captured images are then processed in the CellSelect software to determine whether wells are empty, contain one cell, or contain multiple cells. This data can then be used to dispense reagents into the single‑cell‑containing wells.
Overview of system
*CellTracker Red or a combination of Hoechst and propidium iodide dyes can be used for cell identification and viability assessment.
|Selection||Chips and reagents (sold separately)|
Gain clarity from complex samples
To address cellular heterogeneity, researchers need an unbiased method to analyze large numbers of single cells, more control over selection of the isolated cells to accelerate downstream processing, and the flexibility to analyze multiple parameters per experiment. The SMARTer ICELL8 Single-Cell System meets these requirements with an integrated and automated platform.
By rapidly isolating and characterizing hundreds of rare and unique cells, you can look beyond the aggregate signal seen with traditional bulk processing. The statistical inferences possible with the SMARTer ICELL8 system greatly expand the opportunities for discovery within any biological system.
Bringing the unparalleled sensitivity of SMART-Seq technology to the SMARTer ICELL8 Single-Cell System generates high-quality RNA-seq data from hundreds of single cells. Libraries prepared using this system have a high number of transcripts and genes, even at low sequencing depths. Furthermore, the library complexities remain similar even upon downsampling. Download our application note on full-length single-cell RNA-seq using the SMARTer ICELL8 system.
Combining the SMARTer ICELL8 system with a 5′-RACE-like approach and SMART technology for next-generation sequencing library preparation allows the capture of the full-length variable regions of TCR-α and -β chains for hundreds of cells. Download our application note on high-throughput single-cell TCR profiling using the SMARTer ICELL8 system.
The SMARTer ICELL8 system provides the flexibility of working with both large and small cell types, including nuclei. Above images are of adult cardiomyocytes dispensed in the nanowells. Cells are stained with Hoechst and propidium iodide. Data kindly provided by Dr. Stefan Günther, Max Planck Institute.
Isolate cells of any size
|Primary cells||Mouse||Bone marrow|
|Primary cells||Mouse||Cardiomyocyte (adult)|
|ESC; Differentiated ESC||Mouse||Embryonic stem cells|
|Ear||Mouse||Inner ear organs|
|FACS-sorted lymphocytes||Human||Bone marrow|
|Fetal cortex||Human||Primary cells|
|Fetal neurons||Human||Fetal brain|
|HSPC||Mouse||Hematopoietic stem cells|
|KU812||Human||Blood, CML myeloblast|
|Lung epithelia||Mouse||Primary FACS-sorted cells|
|Nasal epithelia||Human||Primary nasal scraping|
|Nuclei||Human||Frozen lung tumor;|
frozen breast cancer
|Planaria SC||Planarium||Stem cells|
|UTHSC||Human||Bone marrow EW-8 Ewing|
Table I. The ICELL8 MSND's large-bore nozzle allows unbiased isolation of any sample. A few of the cell types that have been isolated using the system are listed. For samples highlighted in blue, the SMARTer ICELL8 Single-Cell System is the first to allow successful isolation of single cells, nuclei, or spheroids.