Single-cell analysis is a powerful method for understanding cellular heterogeneity. Having a high-throughput method to isolate nuclei or single cells of any size, and the flexibility to analyze multiple parameters in single experiments is highly desired for downstream applications like next-generation sequencing. High-throughput single-cell experiments are typically carried out in droplets, fluidic chambers, or nanowells. Regardless of the method it is crucial to be able to distinguish true single cells from multiple cells, and live cells from dead cells, to make meaningful conclusions from the data.
The SMARTer ICELL8 Single-Cell System is an advanced automation platform that combines the power of imaging with the isolation of single cells in 5,184 nanowells. In contrast with other platforms, the SMARTer ICELL8 system employs a large-bore nozzle dispenser for unbiased cell size isolation. Imaging allows the differentiation of single cells from multiple cells. Additionally, with the use of fluorescence, imaging can also be used for live/dead analysis in conjunction with the use of cellular dyes. The system comes with integrated CellSelect software that analyzes the images and provides the ability to proceed only chosen wells, eliminating the noise from multiple-cell-containing wells or empty wells.
The SMARTer ICELL8 platform allows parallel processing of up to eight samples, making it suitable for performing replicates and eliminating batch effects. While the system comes validated with various applications, it is an open platform that allows the user to develop their own applications of choice.