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cDNA Synthesis & Library Construction


SMARTer PrepX reagents for simple, automated NGS library prep

SMARTer PrepX kits provide every reagent necessary for Illumina sequencing library prep in prealiquoted, color-coded, single-use reagent strips—making SMARTer Apollo deck setup effortless. SMARTer PrepX kits and scripts are designed with flexibility in mind to accommodate a variety of sample throughputs. Setup is simple: follow the prompts on the graphic user interface that tell you exactly where and how to place the reagents, minimizing error and stress. Just set up the deck, hit Run, and walk away.

  At-A-Glance   Documents

Features

  • Prealiquoted, color-coded, single-use reagent strips
  • Generate reproducible, high-quality NGS libraries, regardless of input concentrations, within a defined range
  • Accommodate a variety of sample throughputs

Applications

  • Seamless solution for consistent DNA-seq, RNA-seq, and ChIP-seq NGS library prep
  • Eliminates the extensive hands-on and labor-intensive workflows required by many protocols

Performance

Sample input amount and quality are important considerations for any sequencing application. Committing too much of your sample to a workflow or getting a low yield means wasted material, delayed experiments, poor results, or insufficient downstream sequencing material. SMARTer PrepX kits and related scripts resolve these issues by generating comparable, high-quality NGS libraries regardless of input concentrations within a defined range (Table I).

Input amount10 ng100 ng
Adapter reads (%)00
Confidently mapped reads (%)96.6196.43
Repetitive reads (%)1.841.80
Low-quality reads (%)00

For the experiment above, high-quality libraries were prepared using the SMARTer PrepX DNA library prep kit on the SMARTer Apollo system from either 10 ng or 100 ng of E. coli genomic DNA. Libraries were sequenced on the Illumina MiSeq® platform (2 x 150 bp).

Input requirements

Each SMARTer PrepX chemistry is compatible with a script with variable throughputs and minimum input requirements, as summarized in Table II.

Cat. #SMARTer Apollo reagent kitCompatible scriptScript throughputMinimum input amount per sample
640101SMARTer PrepX Complete ILMN DNA Library Kit, 24 SamplesPrepX ILM 81–81 ng DNA
640102SMARTer PrepX Complete ILMN DNA Library Kit, 96 SamplesPrepX ILM 32i4–321 ng DNA
640096SMARTer PrepX RNA-Seq for Illumina Library Kit, 24 SamplesPrepX mRNA 81–8100 pg poly A-enriched RNA; 2 ng rRNA-depleted mRNA
640097SMARTer PrepX RNA-Seq for Illumina Library Kit, 48 SamplesPrepX mRNA 486–48100 pg poly A-enriched RNA; 2 ng rRNA-depleted mRNA
640098SMARTer PrepX PolyA mRNA Isolation Kit, 96 SamplesPrepX PolyA 8 or PrepX PolyA 481–8 or 6–48100 ng total RNA
640099SMARTer PrepX Small RNA for Illumina Library Kit, 24 SamplesPrepX small RNA 81–8100 ng total RNA
640100SMARTer PrepX Small RNA for Illumina Library Kit, 48 SamplesPrepX small RNA 486–48100 ng total RNA

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price Units Select
640096 PrepX™ RNA-Seq for Illumina Library Kit, 24 Samples (400039) 24 samples $2,040.00
640097 PrepX™ RNA-Seq for Illumina Library Kit, 48 Samples (400046) 48 samples $4,080.00
640098 PrepX™ PolyA mRNA Isolation Kit, 96 Samples (400047) 96 samples $672.00
640099 PrepX™ Small RNA for Illumina Library Kit, 24 Samples (400073) 24 samples $2,040.00
640100 PrepX™ Small RNA for Illumina Library Kit, 48 Samples (400074) 48 samples $4,080.00
640101 SMARTer® PrepX™ DNA Library Kit (400075) 24 samples $1,320.00
640102 SMARTer® PrepX™ DNA Library Kit (400076) 96 samples $4,750.00
 

 

 
 

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