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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  NGS_Automation >  Library_Prep_Automation >  Automated_NGS_Library_Prep_Kits

Takara Bio Chemistries for the SMARTer Apollo System

The SMARTer Apollo library prep system combines automated precision liquid handling with gold-standard chemistries, such as SMART-Seq v4 and SMARTer ThruPLEX, to deliver the reliability, flexibility, and reproducible results you expect. Preprogrammed scripts, including those validated for running the SMART-Seq v4 reagent kit or a SMARTer ThruPLEX kit on the system, contribute to significantly reduced hands-on time compared to manual library prep methods: simply set up, hit run, and walk away to perform other tasks.

SMART-Seq v4 Reagent Kit for the SMARTer Apollo System

This kit is designed to generate high-quality, full-length cDNA directly from 1–1,000 cells or 10 pg–10 ng of total RNA using a high-throughput workflow that enables processing of up to 96 samples in a single working day. With this kit, we have reformatted our best-in-class method for ultra-low-input RNA-seq library prep for automation on the SMARTer Apollo platform, providing a walkway solution that performs comparably to manual workflows. cDNA produced with this kit can be processed into NGS libraries suitable for analysis on Illumina® or Ion Torrent platforms. The kit incorporates the same technologies and chemistries associated with our other SMART-Seq v4 products, including oligo(dT)-priming, SMART template-switching technology, and locked nucleic acids (LNA), ensuring the production of cDNA libraries that provide full-length sequence information, accurate representation of GC-rich transcripts, and industry-leading sensitivity.

SMARTer ThruPLEX DNA Library Prep on the SMARTer Apollo System

We adapted our trusted ThruPLEX chemistry for automation on the SMARTer Apollo system, enabling you to start with 50 pg–30 ng of fragmented double-stranded DNA or 1–30 ng of cfDNA and efficiently process up to 48 samples per run, without wasting reagents. This versatile kit, combined with the convenience and consistency of the SMARTer Apollo system, provides added sensitivity to better understand genetic variations within fragmented DNA samples from biofluids such as cell-free DNA, genomic DNA, DNA from FFPE materials, cDNA, or ChIP DNA.Once purified and quantified, the resulting library is ready for Illumina NGS instruments using standard Illumina sequencing reagents and protocols.

  At-A-Glance   Documents

Features

  • SMART-Seq v4 Reagent Kit for the SMARTer Apollo System
    • Industry-leading performance—obtain an accurate representation of rare and GC-rich transcripts
    • Flexible inputs—generate high-quality cDNA libraries from 1–1,000 cells or 10 pg–10 ng of total RNA
    • Walkaway automation—minimize hands-on time and sample-handling errors using SMARTer Apollo scripts developed specifically for this protocol
    • High-throughput sample preparation—obtain purified cDNA from up to 96 samples in a single working day
  • SMARTer ThruPLEX DNA Library Prep on the SMARTer Apollo System
    • Unique, ligation-free method—ThruPLEX technology uses stem-loop adapters to construct high-quality libraries in a fast and efficient workflow
    • Flexible inputs—generate Illumina-ready libraries from 50 pg–30 ng of fragmented double-stranded DNA (gDNA, FFPE DNA, cDNA, or ChIP DNA) or 1–30 ng of cfDNA
    • Walkaway automation—minimize contamination and hands-on time using SMARTer Apollo scripts developed specifically for this protocol

Applications

  • SMART-Seq v4 Reagent Kit for the SMARTer Apollo System
    • High-throughput cDNA synthesis from low-input samples consisting of 1–1,000 cells or 10 pg–10 ng of total RNA
    • mRNA-seq on Illumina or Ion Torrent platforms
  • SMARTer ThruPLEX DNA Library Prep on the SMARTer Apollo System
    • High-throughput, Illumina-ready NGS library preparation from 50 pg–30 ng of fragmented double-stranded DNA (gDNA, FFPE DNA, cDNA, or ChIP DNA) or 1–30 ng of cfDNA
    • DNA-seq and Plasma-seq on Illumina platforms

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Contents Size Price License Units Select
  R400492 ThruPLEX® Plasma-seq 96D Kit 96 Rxns $3,888.00 License Statements
640170 SMART-Seq® v4 Reagent Kit for the SMARTer™ Apollo™ System 96 Rxns $5,964.00 License Statements
 

 

Required Products
Cat. # Product Contents Size Price # of Units Select
640082 SMARTer™ Apollo™ 0.2 ml PCR 8-Tube Strips, Clear (300019) 125 Strips $338.00
640084 Apollo™ Filter Tips (300027) 5 Racks @ 960 Tips Each $600.00
640086 Apollo™ Caps for 0.2 ml PCR 8-Tube Strips, Clear (300029) 125 caps $75.00
640087 Apollo™ Reservoirs (300031) 100 reservoirs $425.00
640078 SMARTer™ Apollo™ Library Prep System 1 each $95,000.00
 

 

 
 

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