Retroviral vectors transduce a wide range of hard-to-transfect dividing cells. Designed for high retroviral titers and high expression of your transgene; compatible with all of our retroviral packaging cell lines.
GP2-293 stably expresses the essential viral packaging genes gag and pol, however, the viral envelope (env) must be supplied in trans via transient transfection. This results in higher titers and the flexibility to choose between envelopes. This cell line is not sold separately, but provided with both of our retroviral packaging systems:
The Retro-X Universal Packaging System includes GP2-293 cells and four different envelope plasmids, so you vary virus tropism (pantropic, i.e. VSV-G, amphotropic, ecotropic, or dualtropic) to suit your target cells.
The Pantropic Retroviral Expression System includes our GP2-293 packaging cell line, the pVSV-G envelope plasmid, and two MMLV-based retroviral expression vectors that allow you to express your gene of interest from alternative promoters.
Increase retrovirus titer 100X—no ultracentrifugation. Just mix your MMLV- or MSCV-based retroviral supernatant with the retrovirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
Perform retroviral transduction in only 30 min using the magnetic beads supplied with the Lenti-X Accelerator. Simply bind your viral supernatant to the beads and pull retrovirus (or lentivirus) to your cells with a magnet.
Coat your culture dish with this multivalent molecule to significantly increase transduction efficiency. RetroNectin co-localizes retrovirus (or lentivirus) particles with your target cell. Perfect for hard-to-transduce suspension cells and stem cells.