Products
|
Viral Transduction
|
|
Selection Guides
|
|
Adenoviral Packaging
|
|
Adenoviral Transduction Tools
|
|
Adenoviral Vector Systems
|
|
AAV Packaging
|
|
AAV Transduction Tools
|
|
|
Resources
|
|
AAV Vector Systems
|
|
Hematopoietic Cell Transduction
|
|
Lentiviral Packaging
|
|
Lentiviral Particles
|
|
Lentiviral Transduction Tools
|
|
|
Lentivirus Titration
|
|
|
Lentivirus Concentration
|
|
Lentiviral Vector Systems
|
|
Retroviral Packaging
|
|
Retroviral Transduction Tools
|
|
Retroviral Vector Systems
|
|
|
|
|

|
|
Products >
Viral_Transduction >
Selection_Guides >
Retroviral_Systems_Selection_Guide
|
|
Retroviral Product Selection Guide
Use the list below to choose the right retroviral product for your research
Retroviral vectors transduce a wide range of hard-to-transfect dividing cells. Designed for high retroviral titers and high expression of your transgene; compatible with all of our retroviral packaging cell lines.
|
Obtain the tightest possible control of gene expression, using Tet-On 3G or Tet-Express systems.
|
Introduce and express target genes in pluripotent cell lines including murine or human hematopoietic, embryonic stem (ES), and embryonal carcinoma (EC) cells.
|
Learn more >>
|
Learn more >>
|
Learn more >>
|
GP2-293 Packaging Cell Line
GP2-293 stably expresses the essential viral packaging genes gag and pol, however, the viral envelope (env) must be supplied in trans via transient transfection. This results in higher titers and the flexibility to choose between envelopes. This cell line is not sold separately, but provided with both of our retroviral packaging systems:
|
The Retro-X Universal Packaging System includes GP2-293 cells and four different envelope plasmids, so you vary virus tropism (pantropic, i.e. VSV-G, amphotropic, ecotropic, or dualtropic) to suit your target cells.
|
The Pantropic Retroviral Expression System includes our GP2-293 packaging cell line, the pVSV-G envelope plasmid, and two MMLV-based retroviral expression vectors that allow you to express your gene of interest from alternative promoters.
|
Learn more >>
|
Learn more >>
|
Alternatively, we offer three cell lines that stably express all three essential packaging genes (gag, pol, and env). These cell lines can be purchased separately.
|
Use the AmphoPack 293 Packaging Cell Line to produce recombinant retroviral particles that infect a broad range of mammalian cells.
|
Use the EcoPack 2-293 Packaging Cell Line to limit tropism to mouse and rat cells.
|
Use the RetroPack PT67 Cell Line to create a stable cell line for continuous retrovirus production.
|
Learn more >>
|
Learn more >>
|
Learn more >>
|
Simply purify viral RNA, and then quantify the number of retroviral genome copies using qRT-PCR and SYBR® technologies.
|
|
Learn more >>
|
|
Increase retrovirus titer 100X—no ultracentrifugation. Just mix your MMLV- or MSCV-based retroviral supernatant with the retrovirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
|
|
Learn more >>
|
|
Perform retroviral transduction in only 30 min using the magnetic beads supplied with the Lenti-X Accelerator. Simply bind your viral supernatant to the beads and pull retrovirus (or lentivirus) to your cells with a magnet.
|
Coat your culture dish with this multivalent molecule to significantly increase transduction efficiency. RetroNectin co-localizes retrovirus (or lentivirus) particles with your target cell. Perfect for hard-to-transduce suspension cells and stem cells.
|
Learn more >>
|
Learn more >>
|
|
|
|