Products
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Viral Transduction
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Selection Guides
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Adenoviral Packaging
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Adenoviral Transduction Tools
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Adenoviral Vector Systems
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AAV Packaging
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AAV Transduction Tools
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Resources
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AAV Vector Systems
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Hematopoietic Cell Transduction
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Lentiviral Packaging
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Lentiviral Particles
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Lentiviral Transduction Tools
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Lentivirus Titration
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Lentivirus Concentration
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Lentiviral Vector Systems
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Retroviral Packaging
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Retroviral Transduction Tools
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Retroviral Vector Systems
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Products >
Viral_Transduction >
Selection_Guides >
Lentiviral_Systems
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Lentiviral Product Selection Guide
Use the list below to choose the right lentiviral product for your research
Learn more about the core elements that we use in all our lentiviral vectors and see schematics of each vector type.
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Express high levels of your transgene from a strong CMV promoter.
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Express your gene of interest from an EF-1 alpha promoter in all cell types, including those in which CMV promoters are often silenced, such as hematopoietic and stem cells.
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Single-vector system to easily generate inducible expression constructs with high fold induction.
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Clone your gene downstream of a ProteoTuner destabilization domain (DD) and rapidly control the level of your protein with Shield1 or Guard1.
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Monitor expression and transduction efficiency with bright red, cyan and green fluorescent proteins.
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Co-express your transgene and an antibiotic or fluorescent marker using IRES-containing lentiviral vector systems.
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Fuse Dmr domains to your protein(s) of interest and induce dimerization with a homo- or heterodimerizer ligand.
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Monitor activity of your favorite promoter using a secreted luciferase.
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Monitor activity of your favorite promoter using a destabilized cyan, green or red fluorescent protein.
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Capture a homogeneous cell population on beads, based on gene expression or promoter activity.
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Obtain titers of >107 IFU/ml. If you are not currently getting at least 107 IFU/ml, you need this HTX system.
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Get the same high titers, but with virus that has an ecotropic envelope. This limits transduction to murine cells or human cells manipulated to express the mCAT1 receptor.
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Produce lentivirus that does not integrate into the target cell genome.
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Not all 293T cell lines are created equal. This one was clonally selected to produce high lentiviral titers.
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Decide if there is enough lentivirus in your culture supernatant, using a simple kit that works like a pregnancy test kit.
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Measure the amount of p24 capsid protein present in your viral supernatant and correlate it directly with lentivirus titer.
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Use a fast and simple quantitative RT-PCR kit to measure lentivirus titer.
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Determine how many lentiviral genomes have integrated into your target cells, e.g. the copy number.
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Increase lentivirus titer 100X—no ultracentrifugation. Just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
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Perform lentiviral transduction in only 30 min using the magnetic beads supplied with the Lenti-X Accelerator. Simply bind your viral supernatant to the beads and pull lentivirus or retrovirus to your cells with a magnet.
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Coat your culture dish with this multivalent molecule to significantly increase transduction efficiency. RetroNectin co-localizes lentivirus (or retrovirus) particles with your target cell. Perfect for hard-to-transduce suspension cells and stem cells.
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Efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise could only infect rodent cells. The booster consists of concentrated exosome-like vesicles that are densely coated with the mCAT-1 receptor protein, the target for ecotropic viruses.
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Simple—just like plasmid maxi-preps. A gentle gravity-flow-based purification procedure is the key to maintaining viral infectivity and producing excellent lentivirus yields of up to 80%.
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