Express your gene of interest from an EF-1 alpha promoter in all cell types, including those in which CMV promoters are often silenced, such as hematopoietic and stem cells.
Get the same high titers, but with virus that has an ecotropic envelope. This limits transduction to murine cells or human cells manipulated to express the mCAT1 receptor.
Increase lentivirus titer 100X—no ultracentrifugation. Just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
Perform lentiviral transduction in only 30 min using the magnetic beads supplied with the Lenti-X Accelerator. Simply bind your viral supernatant to the beads and pull lentivirus or retrovirus to your cells with a magnet.
Coat your culture dish with this multivalent molecule to significantly increase transduction efficiency. RetroNectin co-localizes lentivirus (or retrovirus) particles with your target cell. Perfect for hard-to-transduce suspension cells and stem cells.
Efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise could only infect rodent cells. The booster consists of concentrated exosome-like vesicles that are densely coated with the mCAT-1 receptor protein, the target for ecotropic viruses.
Simple—just like plasmid maxi-preps. A gentle gravity-flow-based purification procedure is the key to maintaining viral infectivity and producing excellent lentivirus yields of up to 80%.
