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Viral Transduction
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Selection Guides
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Adenoviral Transduction Tools
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Adenoviral Vector Systems
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Lentiviral Packaging
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Lentiviral Transduction Tools
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Lentivirus Titration
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Lentiviral Vector Systems
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Retroviral Packaging
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Retroviral Transduction Tools
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Retroviral Vector Systems
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Products >
Viral_Transduction >
Selection_Guides >
Lentiviral_Systems
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Selection Guide - Optimized Lentiviral Vectors and Expression Systems
Lentiviral Product Selection Guide
Use the list below to choose the right lentiviral product for your research
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Learn more about the core elements that we use in all our lentiviral vectors and see schematics of each vector type.
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Express high levels of your transgene from a strong CMV promoter.
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Express your gene of interest from an EF-1 alpha promoter in all cell types, including those in which CMV promoters are often silenced, such as hematopoietic and stem cells.
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Obtain the tightest possible control of gene expression, using Tet-On 3G or Tet-Express.
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Clone your gene downstream of a ProteoTuner destabilization domain (DD) and rapidly control the level of your protein with Shield1 or Guard1.
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Monitor expression and transduction efficiency with bright red, cyan and green fluorescent proteins.
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Co-express your transgene and an antibiotic or fluorescent marker using IRES-containing lentiviral vector systems.
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Clone your shRNA downstream of a U6 promoter to combine high delivery efficiency with knockdown of gene expression.
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Fuse Dmr domains to your protein(s) of interest and induce dimerization with a homo- or heterodimerizer ligand.
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Monitor activity of your favorite promoter using a secreted luciferase.
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Monitor activity of your favorite promoter using a destabilized cyan, green or red fluorescent protein.
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Capture a homogeneous cell population on beads, based on gene expression or promoter activity.
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Obtain titers of >107 IFU/ml. If you are not currently getting at least 107 IFU/ml, you need this HTX system.
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Get the same high titers, but with virus that has an ecotropic envelope. This limits transduction to murine cells or human cells manipulated to express the mCAT1 receptor.
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Produce lentivirus that does not integrate into the target cell genome.
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Not all 293T cell lines are created equal. This one was clonally selected to produce high lentiviral titers.
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Decide if there is enough lentivirus in your culture supernatant, using a simple kit that works like a pregnancy test kit.
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Measure the amount of p24 capsid protein present in your viral supernatant and correlate it directly with lentivirus titer.
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Use a fast and simple quantitative RT-PCR kit to measure lentivirus titer.
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Determine how many lentiviral genomes have integrated into your target cells, e.g. the copy number.
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Increase lentivirus titer 100X—no ultracentrifugation. Just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
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Perform lentiviral transduction in only 30 min using the magnetic beads supplied with the Lenti-X Accelerator. Simply bind your viral supernatant to the beads and pull lentivirus or retrovirus to your cells with a magnet.
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Coat your culture dish with this multivalent molecule to significantly increase transduction efficiency. RetroNectin co-localizes lentivirus (or retrovirus) particles with your target cell. Perfect for hard-to-transduce suspension cells and stem cells.
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Simple—just like plasmid maxi-preps. A gentle gravity-flow-based purification procedure is the key to maintaining viral infectivity and producing excellent lentivirus yields of up to 80%.
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