Retroviral vectors that express shRNA for gene knockdown are available with a constitutive U6 promoter with or without a fluorescent protein reporter. For inducible expression of shRNA see our tetracycline inducible shRNA product page.
RNAi-Ready pSIREN-RetroQ Vectors
RNAi-Ready pSIREN-RetroQ Vectors are retroviral expression vectors based on our Q-Series of retroviral vectors, which are designed to constitutively express an shRNA from the human U6 promoter. These systems are ideal for shRNA delivery into hard-to-transfect cells. Package your RetroQ vector using the Retro-X Universal Packaging System to generate the optimum retrovirus for your cell type.
These vectors are available with Living Colors Fluorescent Protein reporters (Cat. Nos. 632455 & 632487).
Efficient Delivery of shRNA Using Retroviral Transduction
Retroviral transduction enables you to deliver shRNA into 100% of of your target cells, which means that you can achieve knockdown of gene expression in the entire cell population rather than a just portion of it. Even the most efficient plasmid transfections leave a percentage of cells untransfected and thus with no gene knockdown. shRNA delivered using virus therefore increases the dynamic range of knockdown experiments.