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Products >  Viral_Transduction >  Retroviral_Vector_Systems >  shRNA

Retroviral Vectors for RNAi—shRNA Gene Knockdown Using Retrovirus

Retroviral vectors that express shRNA for gene knockdown are available with a constitutive U6 promoter with or without a fluorescent protein reporter. For inducible expression of shRNA see our tetracycline inducible shRNA product page.

RNAi-Ready pSIREN-RetroQ Vectors

RNAi-Ready pSIREN-RetroQ Vectors are retroviral expression vectors based on our Q-Series of retroviral vectors, which are designed to constitutively express an shRNA from the human U6 promoter. These systems are ideal for shRNA delivery into hard-to-transfect cells. Package your RetroQ vector using the Retro-X Universal Packaging System to generate the optimum retrovirus for your cell type.

These vectors are available with Living Colors Fluorescent Protein reporters (Cat. Nos. 632455 & 632487).

Efficient Delivery of shRNA Using Retroviral Transduction

Retroviral transduction enables you to deliver shRNA into 100% of of your target cells, which means that you can achieve knockdown of gene expression in the entire cell population rather than a just portion of it. Even the most efficient plasmid transfections leave a percentage of cells untransfected and thus with no gene knockdown. shRNA delivered using virus therefore increases the dynamic range of knockdown experiments.

At-A-Glance Documents Images & Data Resources

Features

  • shRNA vectors for gene silencing studies
  • Transduction into a broad range of dividing cell types with high efficiency
  • Fluorescent proteins for quick monitoring of transfection efficiency
  • For inducible expression of shRNAs, please see our tetracycline inducible shRNA product page

Applications

  • Gene knockdown in hard-to-transfect cells
  • Delivery to 100% of your target cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
630925 Knockout™ Tet RNAi System H Each $827.00 License Statements *
630926 Knockout™ Tet RNAi System P Each $827.00 License Statements *
631526 RNAi-Ready pSIREN-RetroQ Vector 20 Rxns $396.00 License Statements
632487 RNAi-Ready pSIREN-RetroQ-DsRed-Express Vector 20 Rxns $419.00 License Statements *
632455 RNAi-Ready pSIREN-RetroQ-ZsGreen1 Vector 20 Rxns $411.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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